Method development and validation of Pioglitazone and Saxagliptin by RPHPLC
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2017, Vol 5, Issue 1
Abstract
Analytical method by using RP-HPLC was developed for the determination of saxagliptin and pioglitazone. Potassium dihydrogen phosphate buffer : methanol (80:20 v/v) was used as mobile phase. The detection wavelength was 272 nm, flow rate of 1ml/min and retention time of Saxagliptin was 2.998 minutes and Pioglitazone was 4.310 minutes. The method was validated and all the parameters were within the limits.
Authors and Affiliations
Bolagani Sarada, K Narendra Kumar Reddy, Bada Pragati Kumar
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