MICELLAR ELECTROKINETIC CHROMATOGRAPHY: AN OVERVIEW
Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 4
Abstract
Micellar electrokinetic chromatography (MEKC) is a useful branch of capillary electrophoresis (CE) that utilizes surfactant above critical micellar concentration (CMC) as pseudo-stationary phase. MEKC can be employed to separate both charged and neutral molecules, individually or simultaneously, including chiral compounds. MECK benefits from high peak efficiency due to electroosmotic flow (EOF) in the separation capillary, compounded with large variety of synthetic surfactants, organic modifiers, temperature and variable separation voltage has made MECK. In this review, we present the introduction of CE, fundamentals of surfactant chemistry as it relates to MEKC, separation principles in MECK including equations involved in calculating separation parameters.
Authors and Affiliations
T. Manjula , G. Nagasowjanya , A. Aitha , V. Uma maheswara Rao
Keywords
Related Articles
- EP ID EP138304
- DOI -
- Views 162
- Downloads 0
HPLC METHOD DEVELOPMENT AND VALIDATION: AN OVERVIEW
High performance liquid chromatography (HPLC) is an analytical technique which is proficient to separate, detect and quantify various drugs and its related degradants. An appropriate mobile phase, column, column temperat...
PHARMACOGNOSTICAL AND PHYTOCHEMICAL EVALUATION OF GOKSHURADI GUGGULU - AN AYURVEDIC POLYHERBAL FORMULATION
Gokshuradi Guggulu a polyherbal Ayurvedic formulation is recommended in the management of Madhumeha. There has been an increase in demand for the Phyto-pharmaceutical products of Ayurveda so a pharmaceutical preparation...
A VALIDATED SIMULTANEOUS ESTIMATION OF DOXYLAMINE SUCCINATE AND PYRIDOXINE HYDROCHLORIDE BY UV – SPECTROPHOTOMETRIC METHOD IN BULK AND FORMULATION
A simple and rapid UV – Spectrophotometric method was developed and validated for the simultaneous estimation of Doxylamine succinate and Pyridoxine HCl in combined dosage forms. The said combination is used in tre...
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION AND STABILITY STUDIES OF THE ESTIMATION OF AGOMELATINE IN TABLET DOSAGE FORM BY RP-HPLC
Agomelatine is a new melatonergic antidepressant with a unique pharmacological action. A stability indicating RP-HPLC method was developed and validated for the determination of agomelatine in active pharmaceutical ingre...
SPECTROPHOTOMETRIC ESTIMATION OF CYCLOPHOSPHAMIDE IN CAPSULE DOSAGE FORM
A spectrophotometric method for the analysis of cyclophosphamide in pure form and in capsule has been developed using 0.1N HCl as solvent. The chromogen exhibited absorption maxima at 263 nm. Beer’s law was obeyed in the...