Mild acid hydrolysis of sphingolipids yields lysosphingolipids: a matrix-assisted laser desorption and ionization time-of-flight mass spectrometry study
Journal Title: Journal of Analytical Bio-Science - Year 2012, Vol 35, Issue 3
Abstract
The mechanisms underlying the generation of lysosphingolipids remain unclear. The present study investigated whether sphingolipids can be de-N-acylated under relatively mild acidic conditions to produce lysosphingolipids. Sphingolipids (ceramide, sphingomyelin, cerebroside, lactosylceramide, trihexosylceramide, globoside and Forssman glycolipid) were dissolved in chloroform/methanol (2:1 v/v) and then treated with 0.5N HCl at room temperature. The hydrolysis products were analyzed using thin layer chromatography and matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry. We found that for all sphingolipid species tested, the relatively mild acid treatment resulted in the production of a de-N-acylated sphingolipid. A time-course study showed that approximately 15% of the sphingomyelin sample was hydrolyzed in the first 24 h of treatment, and that hydrolysis continued at a slower rate for 3 weeks thereafter. It appeared that sphingolipid deacylation was induced by cyclic elimination of a fatty acid due to N-acyl to O-acyl conversion. The present findings indicate that lysosphingolipids can be generated from sphingolipids under relatively mild acidic conditions.
Authors and Affiliations
Hiroya Hidaka
The usefulness of validation support reagent in a routine test
In a clinical test, validation is indispensable in order to guarantee the reliability of measurement values and the most fundamental point to be checked. A "Guideline for the validation of quantitative measurement metho...
AMT project for the derivation of reference intervals in Japan
To obtain reliable laboratory test results anytime or anywhere, the JAMT (Japanese Association of Medical Technologists) and the JCCLS (Japanese Committee for Clinical Laboratory Standards) have been proceeding with a re...
Effect on Quality of Life of a Lentinus edodes mycelia-enriched diet (L・E・M®) in patients with terminal cancer
Purpose: It is important to offer the highest possible quality of life (QOL) for terminal cancer patients in palliative medicine. It was examined whether Lentinus edodes mycelia (L・E・M®) -containing diet (CORELEM®) was e...
The degree of oxidative stress in patients with rheumatoid arthritis (RA): The introduction of the ''GAP Ratio'' yardstick and its applications - A ''sampled studies'' approach -
The DAS28 scoring system has long been used as a measure for assessing the condition of rheumatoid arthritis (RA). Disease assessment through good physician-patient collaboration seems to have become an established pract...
Oxidative stress and DNA damage in diabetic nephropathy
Type 2 diabetics show a prevalence rate of 40% for diabetic nephropathy (DN). DN is a devastating complication of diabetes mellitus and a major cause of end stage renal failure. Oxidative stress has been known to play an...