Minimum polyethylene glycol 6000 concentration for selecting Musa spp. in vitro plants tolerant to water stress
Journal Title: Biotecnología Vegetal - Year 2017, Vol 17, Issue 2
Abstract
Increasingly intense droughts are an abiotic factor which has affected bananas and plantains (Musa spp.) production, therefore it is necessary to obtain tolerant genotypes. Plants in vitro selection offers advantages over field selection as it saves time, resources and permit to work with larger plant volumes. Before to establish an in vitro selection protocol for drought, it is necessary first, to establish some conditions. The aim of this work was to determine the minimum polyethylene glycol 6000 concentration which morpho-physiological variables are affected in in vitro Musa spp. plants, ‘Grande naine’ (AAA) and ‘Pelipita’ (ABB) cultivars. In vitro plants were subjected to four polyethylene glycol 6000 concentrations (3, 5, 7 and 9%; w/v) in semisolid multiplication culture medium. Also it was taken into account a control treatment without polyethylene glycol 6000. As stress indicators, plant height, leaf area, fresh weight, dry weight and malondialdehyde content were evaluated. After 30 days of culture, all variables were significantly affected in ‘Grande naine’ cultivar from 3% polyethylene glycol 6000, whereas in ‘Pelipita’ cultivar the affections began to be significative from 5%. These results indicated that ‘Pelipita’ cultivar tolerated the stress better than ‘Grande naine’. From this, using these two cultivars as reference standards, the minimum polyethylene glycol 6000 concentration to select in vitro Musa spp. plants, tolerant to water deficit, may be 3%.
Authors and Affiliations
Leonardo J Moreno-Bermúdez, Maritza Reyes, Rafael Gómez-Kosky, Borys Chong-Pére z
Keywords
Related Articles
- EP ID EP683034
- DOI -
- Views 158
- Downloads 1
Effect of explant type and immersion frequency on potato microtubers cv. ‘Andinita’ production in Temporary Immersion Systems
The potato (Solanum tuberosum L.) is one of the most important crops. Commercial production of microtubers in temporary immersion systems could represent a qualitative step in the production of planting material. This s...
Effect of hydric stress induced by PEG 6000 on water content of Musa spp. ‘Grande naine’ (AAA) and ‘Pelipita’ (ABB) in vitro plants
Bananas and bananas are grown in the tropical and subtropical regions of the world, by hence they are adapted to good humidity conditions. The productivity of these crops is affected by abiotic factors such as drought,...
Effect of parameters involved in the transformation via Agrobacterium tumefaciens in the regeneration of Phaseolus vulgaris L. cultivars ‘ICA Pijao’ and ‘BAT 93’
Phaseolus vulgaris is a crop widely used in human consumption. Its production is affected by the incidence of biotic and abiotic factors. Genetic transformation provides the possibility of reducing these affections, bu...
Behavior of different potato genotypes against early blight(Alternaria solani) (Sor.) in seedbed conditions
The behavior of variantees IBP-10, IBP-27, the varieties ‘Diacol capiro’, ‘Desirée’ and Solanum chacoense species was evaluated in seedbed in front of Early blight diseases. Artificial inoculation with micelial homogeni...
Effect of sucrose concentration and growth regulators in the in vitro tuberization of Dioscorea alata L. variety Cartagena
Among the species of Dioscorea which tubers are used as human food, D. alata L. is one of the most productive and important economically. In this work the effect of sucrose (0, 2, 4, 6, 8,10%) and growth regulators conce...