miRNA-146a-5p is upregulated in serum and cartilage samples of patients with osteoarthritis
Journal Title: Polish Journal of Surgery - Year 2019, Vol 91, Issue 3
Abstract
Introduction: Osteoarthritis (OA) is a widely prevalent joint disease leading to motor disability and pain. Appropriate indicators for identifying patients at risk for this progressive disease, identifying molecular events for detecting early phases of the disease, or biomarkers to screen for treatment responses, however, are lacking. Micro RNAs (miRNAs), which play crucial roles in OA, could be potential biomarkers of OA. Because circulating miRNA levels reflect the disease state, they may be useful for OA screening and as diagnostic tools, reducing the need for invasive procedures and minimizing the cost of current diagnostic methods. Materials and methods: The expression levels of 18 microRNAs (let-7e-5p, miR-21-5p, miR-93-5p, miR-101-3p, miR-103a-3p, miR-130a-3p miR-146a-5p, miR-16-5p, miR-193b-3p miR-199a-3p, miR-210-3p, miR-222-3p, miR-22-3p, miR-27a-3p, miR-27b-3p, miR-335-5p, miR-454-3p, and miR-98-5p) were analyzed by quantitative real-time polymerase chain reaction in the cartilage tissues and serum samples of 28 OA patients and were compared to those of 2 healthy controls. Results: Expression of microRNA-146a-5p was significantly upregulated in the cartilage (p=0.006) and serum (p=0.002) of OA patients. The expression levels of miR-146a-5p in the serum were positively correlated with those in the cartilage (Pearson correlation coefficient R=0.32; p=0.002). Conclusion: miR-146a-5p was significantly overexpressed in patients with OA, both in the articular cartilage tissue and serum, with a positive correlation between the levels in both types of samples. Therefore, the miR-146a-5p serum level could reflect the molecular processes in the cartilage, suggesting its clinical utility as a biomarker for OA management. Implementing noninvasive biomarker using serum miRNAs involves the analysis of the misregulated miRNAs linked to the cartilage pathology. <br/><br/>
Authors and Affiliations
Marzena Skrzypa, Dariusz Szala, Natalia Gablo, Joanna Czech, Justyna Pajak, Marta Kopanska, Mateusz Trzeciak, Krzysztof Gargasz, Sławomir Snela, Izabela Zawlik
Keywords
Related Articles
- EP ID EP517801
- DOI 10.5604/01.3001.0013.0135
- Views 111
- Downloads 0
Zaburzenia czynności seksualnych u pacjentów z gruczolakorakiem odbytnicy po przedniej resekcji odbytnicy
Wprowadzenie: Przednia resekcja odbytnicy (LAR) jest leczeniem z wyboru u pacjentów z rozpoznanym rakiem odbytnicy. Operacja ta ściśle wiąże się z manipulacją w pobliżu narządów moczowo-płciowych oraz związanych z nimi n...
Local Antibiotic Therapy in Rectal Cancer Surgery
Przeprowadzone analizy porównawcze służą powiększeniu aktualnego stanu wiedzy z zakresu dynamiki systemu fluwialnego w krajobrazie nizinnym. Dolina Narwi z terenami bezpośrednio przyległymi rozwinęła się w czwartorzędowy...
Splenic abcesses as infectious complication following implantation of left ventricular asssist device - case report
Left ventricular assist device (LVAD) is one of the modern management therapies in patients with advanced heart failure, and it serves as a bridge to heart transplantation or even as destination therapy. However, it is b...
Alloplastyka śródotrzewnowa w połączeniu z techniką rozdzielenia warstw powięziowo-mięśniowych powłok w olbrzymich przepuklinach brzusznych
Zastosowanie dostępu przedniego w celu oddzielenia komponentów/powłok ściany jamy brzusznej w połączeniu z alloplastyką nadpowięziową („onlay”) (ACST + onlay) w olbrzymich przepuklinach brzusznych (IH) może stanowić wyzw...
Conservative surgery for pancreatic neck transection
The study presented a case of a gastrocutaneous fistula, as a result of bariatric sleeve gastrectomy. The discussion considered the main pathogenesis, etiology, diagnostics and endoscopic treatment using the implantation...