MMP3 -1171 5A/6A Promoter Polymorphism Affects Level of Serum Major Histocompatibility Complex Class Chain Related B in Breast Cancer Patients
Journal Title: International Journal of Medical Laboratory - Year 2017, Vol 4, Issue 4
Abstract
Background and Aims: Involvement of matrix metalloproteinases 3 (MMP3) in breast cancer tumor progression and metastasis has been revealed. MMP3 -1171 5A/6A and 5A/5A polymorphisms in the gene promoter increase expression of the enzyme. The possible relationship of these polymorphisms and serum levels of major histocompatibility complex class I chain-related protein A and B (MICA/B) in breast cancer patients was goal of the study. Materials and Methods: In this case-control study, 105 breast cancer patients and 100 age-matched healthy women were selected. The MMP3-1171 5A/6A polymorphism was determined by nested and restriction fragment length polymorphism polymerase chain reaction. Concentration of MICB and MICA in serum of breast cancer patients has measured using enzyme-linked immunosorbent assay method. Results: Our results showed that 5A/5A + 6A/5A genotypes in the breast cancer group (77.18%) were more frequent compared to healthy participants (64%). However, the frequency of MMP3 -1171 5A/6A genotypes in the breast cancer patients and healthy donors was similar. Additionally, in the patients with 5A/6A genotypes, the mean of MICB concentration was 51.3±16 pg/ml whereas; in the homozygote 6A/6A genotype, it was 4.7±2.6 pg/ml. Statistical analysis confirmed a significant difference between MMP3 -1171 5A/6A and serum MICB levels in the patients (p=0.02). Based on our results MMP3-1171 5A/6A polymorphism did not alter MICA concentration in the patients (p=0.15) Conclusions: The MMP3 -1171 5A/6A genotype might be associated with shedding of MICB in breast cancer patients.
Authors and Affiliations
Ali Shams, Mohammad Hasan Bargostavan
Keywords
Related Articles
- EP ID EP256545
- DOI -
- Views 94
- Downloads 0
The Relationship between Thyroid Hormones, Vitamin D and Fasting Blood Glucose Level During Summer and Winter in Nondiabetic Adults
Background and Aims: 25-hydroxy vitamin D3 is recognized to be an essential element for healthy body; however, its deficiency has been identified as a risk factor for thyroid disease, diabetes mellitus and other autoimmu...
Detecting Virulence Factors and Antibiotic Resistance Pattern of Trueperella Pyogenes Isolated from Bovine Mastitic Milk
Backgrounds and Aims: Mastitis is a mammalian disease which is considered important due to its potential economic damages. Trueperella pyogenesis is one of the important opportunistic pathogens of the mammary glands of c...
Paradoxical Regulation of Copper and Zinc and Changes in Neurogenesis, Alcohol Preference and Salt Appetite in Isolated Male Rats
Background and Aims: Alcohol abuse is an important concern of many societies. Hippocampal neurogenesis regulates abusing drugs in a positive manner. The aim of this study was to identify factors that regulate neurogenesi...
Comparative Effect of Cinnamon Essential Oil, Diclofenac and Morphine on Acute and Chronic Pain in Mice
Backgrounds and Aims: Cinnamomum Zeylanicum is a medicinal herb used in Iranian traditional medicine as an analgesic spice, which its analgesic effect has been experimentally confirmed. Thus, this study was conducted to...
Investigating Class I, II and III Integrons in Multidrug Resistance in Pseudomonas aeruginosa Isolated from Hospital Infections in Ahvaz
Background and Aims: The indiscriminate use of antibiotics can lead to antibiotic resistance in the treatment of infections caused by bacteria such as Pseudomonas aeruginosa. The presence of integrons in Pseudomonas is c...