Modified slide culture method for drug susceptibility testing for Mycobacterium tuberculosis
Journal Title: Indian Journal of Microbiology Research - Year 2017, Vol 4, Issue 3
Abstract
Introduction: Current methods for drug susceptibility testing of Mycobacterium tuberculosis are either costly or slow. As the prevalence of multidrug-resistant strains increases, the need for fast, reliable, and inexpensive methods that can also be applied in settings with scarce resources is obvious. Aim: To determine the prevalence of multi drug resistant tuberculosis by a simple, rapid and cost effective technique. Materials and Method: 100 sputum samples from sputum smear positive patients at RNTCP centre were taken and subjected to drug susceptibility testing for all the first line anti tubercular drugs by modified slide culture method. Samples were also subjected to conventional culture on LJ media. Results: From the 100 smear positive sputum samples, culture of M. tuberculosis was obtained by both LJ medium and modified slide culture for all the samples. Out of the 100 samples, 94 were sensitive for all the drugs, 04 were found to be multidrug resistant and 02 resistant only to isoniazid. Conclusions: The modified slide culture technique was found to be a safe, rapid and cost effective technique and can used for drug susceptibility testing especially in resource limited settings.
Authors and Affiliations
Sumanta A, Sudhindra KS, Nagarathnamma T, Shubha DS
Keywords
Related Articles
- EP ID EP295153
- DOI 10.18231/2394-5478.2017.0067
- Views 84
- Downloads 0
Comparative Study of Dry Cord Care versus Application of 5% Povidone-Iodine on Umbilical Cord in Newborns
Introduction: Infections are the most important cause of infant mortality, of which, umbilical cord infections are an important precursor. In developing countries, umbilical cord infections constitute a major source of n...
Study of extended spectrum beta lactamase producing uropathogens and their antibiotic susceptibility pattern
ntroduction and Objectives: Escherichia coli and Klebsiella pneumoniae are major uropathogenic isolates producing Extended spectrum beta lactamases (ESBL) which confer high resistance to common antibiotics. This study wa...
Characterization of Methicillin Resistant Staphylococcus aureus based on its virulence factors and antimicrobial susceptibility profile
Staphylococcus aureus remains as one of the most potent bacterial human pathogen because of its expression of various virulence factors and also due to its property of multidrug resistance. A total of 468 non –duplicate...
Seroprevalence of Rubella among Female Healthcare Students in a tertiary care teaching hospital
Introduction: Rubella is a disease caused by Rubella virus which can affect anyone of any age and is generally a mild disease, rare in infants or those over the age of 40. Rubella is notorious for causing congenital rube...
Clinico - mycological study on superficial fungal infections in tertiary care hospital and a profile of their antifungal susceptibility pattern
Background: Superficial fungal infections are one among the most common fungal diseases in the world. Dermatophytosis is common in tropical countries like India and may reach epidemic proportions in areas with high rate...