Molecular characterization of some food borne pathogens in soft cheese samples collected from Jeddah, Saudi Arabia
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 3
Abstract
The growing industrial attention toward rapid methods and the wide use of nucleic acid amplification techniques has led for developing and applying of PCR based methods for food-borne pathogens recognition. In the current study, 20 cheese samples, collected from a local supermarket of Jeddah, Saudi Arabia were examined for the presence of metals and some pathogenic bacteria. Cheese content of Na+ and K+ were found to be higher in fresh cheese than other elements. Cr2+, Ni2+, Zn2+ and Al3+ were detected at low concentrations in cheese. Additionally a multiplex PCR method was developed for detection of Salmonella spp., Listeria monocytogenes and Escherichia coli O157:H7 as the most common cheese borne pathogens. Bacterial enrichment was carried out and bacterial genomic DNA was extracted. A set of primers was designed based on specific genes for Salmonella spp. (invA), L. monocytogenes (prfA) and E. coli O157:H7(eaeA). Additionally, a universal –multiplex PCR based on the highly conserved sequences published on genbank database for the previous genes was used for detecting the previous cheese borne pathogenic bacteria. Three (15 %) out of 20 cheese samples, were contaminated with pathogenic bacteria. Finally, the used method is a promised method, simple, rapid and efficient for detecting pathogenic bacteria in contaminating cheese.
Authors and Affiliations
Sanaa Tork1, 2, Safa Qutub Alfattani1, Tahani Mansour Al-Kahtani1, Madeha N. Al-Seeni, Magda M. Aly1, 3
Keywords
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- EP ID EP388938
- DOI 10.9790/3008-1203022734
- Views 70
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