MOLECULAR CHARACTERIZATION OF THE 16S RRNA GENE OF HELICOBACTER PYLORI ISOLATED FROM STOMACH BIOPSY IN GASTRITIS PATIENTS
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2013, Vol 2, Issue 5
Abstract
Background: Helicobacter pylori is now accepted as the most important cause of gastritis in human. There is a growing body of literature on the detection of H. pylori 16S gene in gastritis patients. The study was aimed to determine the frequency of H .pylori (16-S) gene, in patients with gastritis at Khartoum State, Sudan. Methods: Molecular testing for H. pylori 16-S gene was done on 40 stomach biopsy using PCR technique. Biopsy specimens were collected by gastroenterologist using endoscopy. One biopsy of inflamed stomach was collected in sterile normal saline and kept at -20OC till used. Extraction was done by using ml jena bioscience kit. The amplification reaction was carried out in thermo cycler machine PCR system with program system consisting of an initial denaturation step at 94 OC for 10 minute and 30 cycle of denaturation at 94 OC for 1 minute Results: Twenty one samples (52.5%) out of 40 were positive by PCR, while 19 samples (47.5) were negative. Conclusion: The frequency of 16 S rRNA genes of H. pylori among endoscopic patients was 52.5%,
Authors and Affiliations
Hiba Nugdalla Allah Abdorahman| B.Sc. Medical Microbiology Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, Al Neelain University, Aymen Awad Abdelhaleem| Ph.D. Department of Molecular Biology & Immunology, Medical Research Center MRC University of Jazan K.S.A, Walid Ahmed Hamid Eldaif| Ph.D. Medical Microbiology Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, Al Neelain University, Mohanad Eltahir Ismail Mosa| B.Sc. Medical Microbiology Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, Al Neelain University, Waad Abdelmonem Elsheikh Ahmed| B.Sc. Medical Microbiology Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, Al Neelain University, Amira Abbas M. Fadl| FRCP London. Almudaris Medical Center Khartoum Sudan
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