Monitoring of Leptosphaeria maculans and L. biglobosa ascospores around East Sudethian mountains – a joint initiative of Poland and the Czech Republic
Journal Title: Rośliny Oleiste - Oilseed Crops - Year 2010, Vol 31, Issue 1
Abstract
A joint initiative of the Polish and Czech research institutes enabled monitoring of Leptosphaeria maculans and L. biglobosa ascospores around East Sudethian mountains — in the regions of intensive oilseed rape cultivation in both countries. Infection by both fungal species leads to blackleg or stem canker, causing considerable yield losses of brassicas. Daily concentrations of ascospores were observed from 1 September to 30 November 2008 at four sites, including two locations in Poland and two in the Czech Republic. The spore monitoring was done using Hirst type volumetric traps. The mean inoculum concentration was calculated on the basis of spore number on microscope slides containing cellophane tapes covered with a film of Vaseline, which enabled trapping and immobilization of spores. The species were identified based on Real-Time PCR data.The first ascospores of L. maculans / L. biglobosa were detected in mid-September, and the maximum concentration of ascospores was observed from 2 to 19 days after the first spore release. At the earliest both parameters were observed at Šumperk, in the Czech Republic. The highest number of propagules per cubic meter ranged from 3 to 10 spores. Ascospores were present for 41.8% to 57.1% of days monitored. The inoculum of L. maculans was found less frequently, but the highest concentrations of DNA of this pathogen greatly outnumbered the values obtained for L. biglobosa. At both sites L. biglobosa was observed more frequently. The inoculum density of L. maculans and L. biglobosa in the autumn of 2008 was smaller as compared to the other seasons, what showed less risk of oilseed rape plant infection by the pathogens causing stem canker of brassicas over the period of this study.
Authors and Affiliations
Małgorzata Jędryczka, Eva Plachká, Joanna Kaczmarek, Jana Poslušná, A. O. Latunde-Dada, Agnieszka Mączyńska
Keywords
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