Monitoring of Marketed Pork of Assam, Meghalaya and Manipur for Fenbendazole Residues
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2017, Vol 1, Issue 3
Abstract
The study was undertaken to monitor marketed pork of Assam and its neighboring states Meghalaya and Manipur for Fenbendazole residues using Ultra High Performance Liquid Chromatography (UHPLC). 675 Samples of marketed pork of Assam, Meghalaya and Manipur were collected for the study. The samples after collection were preserved at -20 C. Recoveries of Fenbendazole in pork ranged from 89-98 %.Out of the tested samples, 29 samples were detected to be positive for trace residues of Fenbendazole which were well below the Maximum Residue Limit (MRL) value. Introduction: Residues of Veterinary drugs are one of the major problems for food contamination. Veterinary drugs residues usually accumulate in the liver or kidney rather than other tissues. Fenbendazole is an anthelmintic frequently used in farm animals to treat parasitic infestations in pig [1]. The most likely reasons for Fenbendazole residues may result from human management, such as improper usage, including extra label or illegal drug applications [2]. The North-Eastern states of India is characterized by a high proportion of tribal people for whom pig rearing is integral to their way of life and pig meat is considered as an important food item. Thus the present study was undertaken for monitoring of Fenbendazole residues in pork samples from various areas of Assam and its neighboring states Meghalaya and Manipur using UHPLC. Methods and Methods: 675 samples of swine tissues comprising of Muscle, Kidney and Liver were collected from different markets of Assam, Meghalaya and Manipur (Table 1). Representative samples weighing 30 g each belonging to same carcass were wrapped in polythene bags and transported in thermo-cooled containers jacketed with ice. The samples were stored at -20 C till the time of processing. The levels of Fenbendazole were determined using a UHPLC system of Make: Dionex with a wavelength at 290 nm as per Method described by Cooper et al. [3]. The samples were separated on a RP-C 18 column and were eluted with a mobile phase of mixture of Water and Acetonitrile in the ratio of 70:30 v/v. The isocratic mode was run at a flow rate of 1 ml/min. Chemical and Reagents: Fenbendazole standard (Dr Ehrenstofer, Germany; HPLC grade Acetonitrile (Fisher), Methanol (Fisher); other chemicals and solvents of analytical grade and HPLC grade water (Fisher) were used for the study. Preparation of sample: The fascia and fat of pork were removed and then cut into small pieces.5 g of the sample was taken in a glass test tube and to it added 2ml of Phosphate buffer and mixed. The mixer was then sonicated by setting at 15 micron amplitude for 20 cycles with a stop time of 30 second interval at low temperature which was maintained with crushed ice. The sonicated sample was then left undisturbed for 15 mins for allowing the extract to dissolve in the solvent.
Authors and Affiliations
DC Roy, R Gogoi
Keywords
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- EP ID EP569566
- DOI 10.26717/BJSTR.2017.01.000250
- Views 178
- Downloads 0
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