Morphological and Histological Effect of Oral Intake of Rose Water in Mouse
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 6
Abstract
Rose water (Maa El-Ward) is one of the major products of rose, it is produced from the petals of Rosa damascene and contains about 0.08-0.12% of essential rose oil. Rose water is used widely between Saudi women (Saudi Arabia), in cooking and taken orally due to its calming effect. This study has been initiated to highlight the morphological and histological changes on mice and its kidney, using two types of rose water (RW1 & RW2), by oral uptake. Eighty four adult female albino mice were divided into one control group (12 mice) that was administered distilled water and two experimental groups. Each experimental group was subdivided into three subgroups 12 each and received oral doses twice/day for eight consecutive weeks under laboratory conditions. The first experimental subgroups were taken RW1 at doses 41, 82 & 174 μl /body weight. The second experimental subgroups were given RW2 at doses 42, 85, & 173 μl /body weight. Every two weeks, blood samples were collected and the sera were analyzed biochemically. Renal tissue samples were also taken and histopathological changes of the kidneys were examined using optical microscope. Biochemical analysis showed significant difference in the serum levels of both creatinine and uric acid between the experimental groups and the control. Varieties of histological changes were recorded in all experimental groups in terms of the intake dose and time interval. Results showed that slight degenerative changes in renal tubules elements of all treated groups, after two weeks of receiving the dose, in comparison with control. These changes increased with time and became more obvious in RW2 than RW1. These histological changes included wide spacing of tubules and atrophy of the lining epithelium. Proximal tubules showed large numbers of shrunken dark acidophilic cells with dark small nuclei. Renal medulla tubules appeared with degenerated cells and vascular congestion. In conclusion, the observed significant increases of serum parameters and histopathological changes in the kidneys of treated mice suggested that rose water may cause nephrotoxicity in mice. So, the results of this study send a public awareness for women who oral intake of rose water as it might cause histological damage to kidney for long term use.
Authors and Affiliations
Nouf Hakami a, Rabah, Samar
Keywords
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