Evaluation of Antidiabetic Activity of Ethanolic Extract of Trema Orientalis (L.) Blume Leaves.
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 5
Abstract
Context: Trema orientalis(L.)Blume is a species of flowering tree in the hemp family Cannabaceae. This common tree is widely used in African folk medicine for many diseases, for example asthma, cough, dysenteria and hypertension Objectives: The objective of the study was to evaluate the antidiabetic activity of ethanolic extract of Trema orientalis (L.) Blume leaves by in vivo methods. Material and methods: The leaves of Trema orientalis were dried under shade and then powdered, and extracted with ethanol by hot continuous extraction method using soxhlet apparatus. Preliminary phytochemical studies were carried out on extract. The antidiabetic potential of ethanolic extract of T.orientalis (EETO) was evaluated by using Streptozotocin-Nicotinamide induced type 2 diabetic rat model. Two doses of the EETO (250mg/kg and 500mg/kg) were administered to normal and experimental diabetic rats for 14 days. The blood samples were withdrawn from the retro orbital sinus on 1, 7 and 14th days of extract administration and fasting blood glucose levels were estimated. In addition, changes in body weight and serum lipid profiles, assessed in the extract treated diabetic rats were compared with diabetic control and normal animals. OGTT and histopathological studies of pancreas were also performed. Results and discussion: The study showed that, extract treatment in diabetic rats at two dose levels showed a decrease in fasting blood glucose levels. The total cholesterol and serum triglycerides levels were also reduced and the HDL cholesterol levels were increased upon treatment with the extract thus proving the potent antidiabetic property of the plant. Recovery of body weight of extract treated diabetic rats gave further evidence for dose related antidiabetic activity of EETO. The extract did not produce any significant effects on normal animals but significantly increases glucose tolerance of normal animals, which further confirms the safety and antidiabetogenic action of the extract. Conclusion:The study confirmed the potent antidiabetic activity of the plant Trema orientalis (L.) Blume.
Authors and Affiliations
Jiji . K. N1, Pramod. C2, Boby S Prasad2, DR. P. Muralidharan1
Keywords
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- EP ID EP385916
- DOI 10.9790/3008-1105011726
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