Morphological study of GABAergic neurons involved in pulpitis pain and related anxiety
Journal Title: Journal of Air Force Medical University - Year 2023, Vol 44, Issue 11
Abstract
Objective To establish a mouse model of experimental pulpitis, detect pain and anxiety-like behaviors, and observe the activation of neurons in insular cortex (IC) and medial prefrontal cortex (mPFC) and the activity changes of GABAergic neurons. Methods The experimental pulpitis model was established and HE staining was used to observe the changes of pulp inflammation. Pain behavior was used to detect the pain degree of pulpitis. Anxiety-like behaviors of mice with pulpitis were detected by elevated plus maze test and open field test. GAD67 -GFP knock-in mice and immunofluorescence staining were applied to observe the distribution of FOS protein expressed by an immediate early gene c-Fos in IC and mPFC and its co-localization with GABAergic neurons. Results After pulpitis modeling, HE staining showed that the inflammation progressed to 1 / 3 of the root at day 3 and 1 / 3 of the root tip at day 7, and the pulp was almost completely necrotic at day 14. The pain threshold decreased 1 d after modeling, and the lowest pain threshold was found on the 7th day. The exploration time in central area in the open field test and the exploration time in open arms in the elevated plus maze test in the pulpitis group were significantly shortened. The number of FOS+ cells increased in IC and mPFC, and co-labeled cells of FOS and GABAergic neurons were observed after 7 d of pulp exposure. Conclusion The mouse pulpitis pain is accompanied with anxiety-like behaviors. In the state of pain, neurons in IC and mPFC are activated, and GABAergic neurons show changes in activity, which provides morphological evidence for the involvement of GABAergic neurons in pulpitis pain and accompanied negative emotions.
Authors and Affiliations
SI Jialin, ZHANG Yaoyuan, KANG Xiaoning, WU You, MA Ningcan, YU Zhuo, HUANG Jing, WU Li‘an
Keywords
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