MPO release reaction against activated neutrophils
Journal Title: Journal of Analytical Bio-Science - Year 2013, Vol 36, Issue 2
Abstract
Against infection, neutrophils form phagosomes in which microorganisms are absorbed by phagocytosis. Then, from the azurophilic granules of neutrophils, Myeloperoxidase (MPO) and granule components are released. In this process, it is suspected that the activation of the NDPH oxidase and de-granulation along with the formation of phagosomes are related to the release of MPO. However, the relationships between the activation of neutrophils and MPO release have not been clarified. Thus, the authors examined the mechanism of the signal transmission at the time of neutrophils activation and MPO release. Concerning the MPO release, fMLP stimulation did not show significant inhibition up to high concentration level by Genistain and GF109203X. The PMA stimulation showed significant concentration-dependent inhibition reaction by GF109203X. These results indicate that the transmission of PKC stimulation acts on the release of MPO. Concerning the mechanism of the MPO release, the MPO expression was examined using flow cytometry with the MPO-labeled antibody. The MPO expression significantly increased with the PMO stimulation, while it did not show increase with the fMLP stimulation. The MPO expression with the PMO stimulation was significantly inhibited by GF109203X. These results indicate that the MPO release occurs in the following order: activation of neutrophils by signal transmission of PKC, de-granulation, release of MPO to phagolysosome, expression in cell membrane and release of MPO outside of the cell.
Authors and Affiliations
Yasuhiko Ohta
Keywords
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