ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF RAMIPRIL HCL AND METOPROLAL TARTRATE IN PURE AND IN COMBINATIONS BY UV SPECTROPHOTOMETRY AND RP-HPLC
Journal Title: International Journal of Pharmaceutical Development & Technology - Year 2014, Vol 4, Issue 4
Abstract
ABSTRACT Simple, rapid and accurate UV Spectroscopic (Simultaneous Equation method and First order derivative method) and an isocratic RP – HPLC methods showed excellent sensitivity, reproducibility, accuracy, and repeatability. In simultaneous UV method, the overlaid spectra of mixture of Ramipril HCl and Metoprolol Tartrate were recorded. From the spectra, 206nm for Ramipril HCl and 222nm for Metoprolol Tartrate was selected as wavelength to construct simultaneous equation. The percentage label claim present in tablet formulation was found to be 101% and 103% for Ramipril HCl and Metoprolol Tartrate respectively. In second method, the same spectrums were derivatised and 205 nm selected for detection of Ramipril HCl where Metoprolol Tartrate shows zero crossing and also 210 nm selected for detection of Metoprolol Tartrate where Ramipril HCl shows zero crossing. The percentage label claim present in formulation was found to be 98.5% and 101% for Ramipril HCl and Metoprolol Tartrate respectively. In RP-HPLC method, mobile phase used is acetonitrile: methanol: acetate buffer pH 5.0 (30:50:20 V/V) with flow rate of 0.9 ml per min, the retention time of Metoprolol Tartrate and were found to be 2.84 and, 3.55respectively. The percentage purity was found to be 99.76% and 99.82% for Metoprolol Tartrate and Ramipril HCl, respectively. The low % RSD values for recovery indicated that the method was found to be accurate. Keywords: Analytical Method Development and Validation, Ramipril, Metoprolal, UV Spectrophotometry, RP-HPLC.
Authors and Affiliations
Gadireddy Sujana*, G. Aruna, G. Sivagangaram
Keywords
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