CAPILLARY ELECTROPHORESIS INDUCTIVELY COUPLED MASS SPECTROMETRY
Journal Title: Int J of Preclin & Pharm Res - Year 2014, Vol 4, Issue 7
Abstract
ABSTRACT Capillary electrophoresis (CE) is a separation technique in which the analytes are separated on the basis of differences in their charge-to-size ratios. This makes CE especially suitable for the analysis of molecules with abroad range of sizes, charge and hydrophobicity, as proteins and peptides. Another attractive feature of CE is its ability to handle minute sample amounts, with nano-litre injection volumes, which makes it ideal for applications when the sample volumes are limited, as often is the case in analysis of body fluids, single cells and other small volume analysis of biofluids. Mass spectrometry (MS), is today one of the most advanced detection techniques. It yields maximum accurate information in short time, consuming minimal amounts of sample. MS provides robustness and reproducibility of analysis as well as high throughput possibilities via automatic data acquisition. When combined with ionization techniques like inductively coupled plasma (ICP), electro spray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI), MS allows simultaneous analysis of proteins and peptides with high resolution. However, in order to reach ultimate mass sensitivity a separation step prior to MS detection is often needed. The possibility of combining the properties of MS with a fast and high-efficiency separation provided by CE to meet the analytical challenges, that the complexity of biological samples represents, has been recognized for about two decades. Keywords: Capillary electrophoresis, ICP, Coupling.
Authors and Affiliations
Shashe Kumar P*, Ramamohan Reddy T, Ajitha A, Umamaheshwar Rao V
Keywords
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