METHOD DEVELOPMENT AND VALIDATION OF RP-HPLC ANALYSIS OF BROMFENAC IN RABBIT PLASMA
Journal Title: Int J of Preclin & Pharm Res - Year 2014, Vol 4, Issue 8
Abstract
ABSTRACT A simple, precise, rapid, and accurate reverse phase high performance liquid chromatography method was developed for the estimation of Bromfenac in Rabbit plasma by using Paracetamol as IS. The protein precipitation technique was used for the extraction of analyte from rabbit plasma. The chromatographic separation was performed on Agilent, Zorbas SB C18 (4.6mm×250mm×5µ) column with PDA detection at 267nm. The mobile phase comprising of mixture of methanol and buffer 0.1% ortho phosphoric acid (pH 2.6) in the ratio of 30:70v/v, at the flow rate 0.8ml/min. The analytical method described here is valid for the estimation of Bromfenac in Rabbit plasma over a range of 50µg/ml-150µg/ml. The retention times of Bromfenac and Paracetamol were found to be 3.781 and 2.470mins respectively. The correlation coefficient for Paracetamol and Bromfenac were 0.998 and 0.999 respectively. The LOD and LOQ values for Bromfenac were found to be 0.13µg/ml and 0.40µg/ml respectively. %RSD of Bromfenac and Paracetamol was obtained to be 0.41and 0.28 respectively. This method is time efficient and samples are easy to monitoring Bromfenac in plasma. Keywords: HPLC-PDA method, Rabbit Plasma, Bromfenac, Protein precipitation.
Authors and Affiliations
Vidya Kamdar*, Uma Maheshwara Rao, Vattikuti, Ajitha Azhakesan
Keywords
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