DIFFERENCE ABSORBANCE SPECTROPHOTOMETRIC DETERMINATION OF OFLOXACIN AND ORNIDAZOLE IN DOSAGE FORM
Journal Title: Int J of Preclin & Pharm Res - Year 2013, Vol 3, Issue 2
Abstract
ABSTRACT Describing the simple, precise and rapid method based on difference absorbance spectroscopic technique for the determination of Ofloxacin (OF) and ornidazole (OZ) in tablet dosage form. The projected method was based upon measuring the difference absorbance of OF at 293.4 nm (λ max of 0.1N HCl) in 0.1N NaOH vs 0.1N HCl solutions and other drug OZ difference absorbance in 0.1 N NaOH vs 0.1 N HCl at 277.9 nm (λ max of 0.1N HCl). The Beer’s law was obeyed in the concentration range of 4-24 ug/mL and 5-30 μg/mL for OF and OZ respectively. The lower limits of detection (LOD) of OF and OZ are 0.31 and 0.35 μg/mL, respectively, while the lower limits of quantification (LOQ) of OF and OZ were 0.92 and 1.04 μg/ mL, respectively. The precision and accuracy of the method was acceptable; the relative standard deviations did not exceed 1.1% (n=6) and recovery was between 98.25% and 100.8% for OF and OZ, respectively. The proposed method was validated and successfully applied for the determination of both drugs in bulk powder, laboratory prepared mixture and commercial dosage forms such as tablets without interference from the commonly encountered excipients and additives. Keywords: Ofloxacin, Ornidazole, Difference spectroscopy, Beer’s law.
Authors and Affiliations
Siva Kumar CHV*, Subash kusum Ray, Meera I
Keywords
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- EP ID EP154218
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