DEVELOPMENT AND VALIDATION OF NEWER ANALYTICAL METHOD FOR THE ESTIMATION OF NEVIRAPINE IN BULK AND IN TABLET DOSAGE FORM BY RP – HPLC METHOD
Journal Title: International Journal of Pharmaceutical Development & Technology - Year 2015, Vol 5, Issue 1
Abstract
ABSTRACT A simple, sensitive, specific RP-HPLC method was developed for the estimation of Nevirapine in bulk and pharmaceutical formulation. This method was based on HPLC separation of the drug in reverse phase mode using C18 column (150 mm × 4.6 mm i.d. 5μ). The mobile phase constituted of Acetonitrile: 0.01M Phosphate Buffer (PH 5.3) adjusted with orthophosphoric acid (40:60 v/v) and flow rate 1.0ml/min. Detection was performed at 220 nm. Separation completed with in 5minutes. Calibration curve was linear with the correlation coefficient was 0.9996 over a concentration range of 50 to 150μg/ml for the drug. The relative standard deviation (R.S.D) was found <2.0% for RP-HPLC method.This method have been successively applied to bulk and pharmaceutical formulation. The present method was validated according to ICH guidelines. Keywords: Nevirapine, UV-spectroscopy, High Performance Liquid Chromatography.
Authors and Affiliations
Prem kumar bichala*1 and A. Lashmana rao2
Keywords
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