Development and Validation of Stability Indicating Method for Simultaneous Estimation of Cefepime and Tazobactam Injection using RP-UPLC Method
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2014, Vol 4, Issue 12
Abstract
This research manuscript describes simple, sensitive, accurate, precise and repeatable RPUPLC method for the simultaneous determination of Cefepime (CEFE) and Tazobactam (TAZ) Injection in combine dosage form. The sample was analyzed by reverse phase C18 column (Acquity UPLC BEH 100 × 2.1 mm ID, 1.7 µm) with mobile phase. In mobile phase, Solution A containing Potassium Dihydrogen Phosphate buffer (pH adjusted to 6.5±0.2 with Orthophosphoric acid), Citric acid buffer (pH adjusted to 5.0±0.2 with NaoH solution) and Acetonitrile and Solution B containing Tetradecyl ammonium bromide, Tetraheptyl ammonium bromide and Acetonitrile in the flow rate of 0.3 ml/min. Quantification was achieved 230 nm with PDA detector. The retention time for Cefepime and Tazobactam was found to be 0.68 and 1.69 minute respectively. The linearity for Cefepime and Tazobactam was obtained in the concentration range of 40-280 µg/ml and 5-35 µg/ml respectively. Cefepime and Tazobactam API and market formulation were subjected to acid and alkali hydrolysis, oxidation, thermal and photolytic forced degradation. The peak purity of drug substance and drug product peak also confirmed the specificity of the methods with respect to the degradation products. In the forced degradation study Cefepime and Tazobactam showed maximum degradation in base hydrolysis stress study followed by less degradation in thermal degradation. The developed method was simple, specific, sensitive, rapid, and economic and can be used for estimation of Cefepime and Tazobactam in bulk and their combined dosage form for routine analysis and stability studies
Authors and Affiliations
Panchal Vipul J , Desai Hemant T 1 , Patel Nirav B 1 , Panchal Kalpesh B 1
Keywords
Related Articles
- EP ID EP137399
- DOI -
- Views 81
- Downloads 0
A Comparative Study of Metformin plus Sulfonylureas Versus Metforminalone for its efficacy in Type 2 Diabetes patients
The objective of the study was to compare the efficacy of combination containing Metformin plus Sulfonylureas (MET+GLI/GLIB) and to determine whether combination of Sulfonylureas had clinically remarkable benefit over Me...
Production and characterization of monoclonal antibodies raised against the membrane ecdysone receptor from the anterior silk gland of the silkworm, Bombyx mori
Several evidences for nongenomic action of ecdysteroids through membrane receptor (mEcR) in programmed cell death (PCD) of the Bombyx mori anterior silk glands (ASGs) have been reported. The isolation, characteri...
Antioxidant activity of polysaccharide from Sargassum sp
Brown seaweeds are widely used in the medical field to treat tumor cells. In this present study brown seaweed Sargassum species was used to isolate polysaccharide and quantitative estimation of carbohydrate was done and...
Eye banking and corneal transplantation in Tertiary care hospital located in rural area
: Considering the magnitude of corneal blindness and shortage of donor material, more marked in rural area this study about eye banking and corneal transplantation was conducted in tertiary care hospital located...
Grain and Milling Quality of Barley and Their Suitability for Preparation of Traditional South Indian Products
Barley (Hordeum vulgare L.) is grown as a commercial crop in some hundred countries worldwide and is one of the most important cereal crops in the world. Barley assumes the fourth position in total cereal produc...