Effect of Pakistani medicinal plants on IgE/antigen- and ionophore-induced mucosal mast cells degranulation
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2014, Vol 27, Issue 4
Abstract
Cumulative evidence has now demonstrated the stimulation of mucosal mast cells by both allergic and non-allergic triggers and their inhibition as a potential therapeutic target in many diseases like food allergy and ulcerative colitis. Hence, we screened medicinal plants from Pakistan against antigen- and ionophore-induced degranulation of mucosal mast cells. Aqueous ethanol extracts were screened. IgE/antigen- and A23187-induced degranulation of mucosal-type murine bone marrow derived mast cells (mBMMCs) were screening assays and -hexosaminidase released from degranulated mBMMCs was measured. Real time-polymerase chain reaction was employed to examine the expression of TNF-and IL-4 mRNA. Acetoxychavicol acetate, was examined by degranulation assays and real time-PCR. Among the ten plants screened against IgE/antigen stimulated degranulation, five plants; Alpinia galangal, Mentha arvensis, Myrtus communis, Polygonum bistorta and Syzygium aromaticum demonstrated significant (p<0.01) suppression of the degranulation at 100 g/ml. Of them, Alpinia galangal showed significant (p<0.01) inhibition at 32 g/ml. In A23187-induced degranulation, all plants showed significant (p<0.01) inhibition at 100 g/ml except Tamarix dioica. Again Alpinia galangal exhibited significant (p<0.01) suppression at 32 g/ml. In a concentration dependent assay, Alpinia galangal revealed significant suppression at 10 g/ml against A23187-stimulated degranulation. Acetoxychavicol acetate demonstrated significant (p<0.01) inhibition at 3.2 M in IgE/antigen-treated cells and at 10 in A23187-treated cells. Furthermore, both Alpinia galangal and acetoxychavicol acetate suppressed the IgE/antigen- and A23187-enhanced mRNA expression of inflammatory cytokines, TNF- and IL-4, in mBMMCs. Our findings revealed the suppressive effect of Alpinia galangal and acetoxychavicol acetate on degranulation of mBMMCs by allergic and non-allergic stimuli, which can be utilized for future drug development against food allergy or ulcerative colitis.
Authors and Affiliations
Syed Faisal Zaidi , Ji-Hyun Kim , Yashiro Tomoe , Khan Usmanghani , Makoto Kadowaki
Keywords
Related Articles
- EP ID EP142102
- DOI -
- Views 117
- Downloads 0
[u][/u] Preliminary evaluation of antimicrobial activity of cream formulated with essential oil of Trachyuspermum ammi
: In our present research we studied physiochemical properties and antimicrobial activity of T. ammi (Trachyuspermum ammi) seeds. The seeds yielded 2.86% essential oil. Refractive index, specific gravity, acid valu...
Study of phenolic content and urease and alpha-amylase inhibitory activities of methanolic extract of Rumex acetosella roots and its sub-fractions in different solvents
The present study aimed to establish relationship between urease and alpha-amylase inhibitory activities on the one hand and on the other between anti-enzymatic activities and total phenolic contents of the methano...
Rapid separation and determination of betulinic acid from a complex matrix using combination of TLC and RP-HPLC
Hitherto, only a few studies are reported about using the combination of TLC and RP-HPLC for the separation and determination of analyte(s) from a complex matrix. The present study is aimed to develop a simple and rapid...
Evaluation the thermodynamic behavior of nonionic polyoxyethylene surfactants against temperature changes
Micellization is the most important property of surface agents. It plays an important role in the manufacture of pharmaceutical products. The surfactants have many applications in industry, agriculture, mining and...
Molecular characteristics of woody extracts of Buxus microphylla
As one of famous shrubs in China, Buxus microphylla is considered as the important wattle. However, the constituents of Buxus microphylla stem extracts aren’t used effectively. Therefore, the molecules of stem extr...