Fluoride effect on the process of alveolar bone repair in rats: evaluation of activity of MMP-2 and 9
Journal Title: RSBO - Year 2012, Vol 9, Issue 3
Abstract
Objective: The aim of this study was to evaluate comparatively the effect of fluoride (F) on the activity of matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9) involved in process of alveolar bone repair. Material and methods: This study used 4 groups of Wistar rats with 80 days of life (n = 160) which received drinking water containing different doses of fluoride (NaF): 5, 15, 50 ppm and deionized water (control) throughout the experiment. These animals had their right upper incisors extracted. After extraction, the animals were euthanized at 7, 14, 21 and 30 days and the hemi-maxillae were collected for microscopic analysis (Hematoxylin and Eosin and immunohistochemistry for MMP-9) and zymography (MMP-2 and 9). Results: Microscopically the process of bone repair was similar in all groups, being noted only a delay of the blood clot resorption and bone formation in the group of 50 ppm F. The expression for MMP-9 showed differences between groups only during the initial repair (7 days). However, the zymography showed no significant differences between treated and control groups. Conclusion: Ours results suggest an effect of fluoride on the activity of MMPs 2 and 9 at the initial period of alveolar repair which could be associated to the process of blood clot remission and delay in bone repair. Further studies are needed to establish the relationship between the initial process of resorption of the blood clot, and the involvement of MMPs 2 and 9 and its regulators/tissue inhibitors.
Authors and Affiliations
Mileni Fernandes, Gisele Martins, Lucas Shimoraha, Flávia Iano, Marcela Yanai, Tânia Cestari, Marília Buzalaf, Rodrigo de Oliveira
Keywords
Related Articles
- EP ID EP98444
- DOI -
- Views 185
- Downloads 0
Consequences of dental trauma to the primary teeth on the permanent dentition
Introduction: Dental trauma to primary teeth or to the jaws may cause disorders on the development of permanent successor teeth. The alterations may either occur at the time of accident or be caused by post-traumat...
Influence of root curvature’s initial position on apical deviation occurrence after oscillatory preparation in simulated root canals
Introduction and objective: This study aimed to analyze the influence of root curvature’s initial position on apical deviation occurrence after oscillatory system preparation. Material and ethods: For this purpose, we us...
Resistência de união ao microcisalhamento entre compósitos restauradores e cimentos resinosos
Introduction and objective: The techniques of adhesive cementation have been widely used in dental restoration. The purpose of this study was to evaluate the microshear bond strength between restorative composites and re...
Gubernacular cord and canal – does these anatomical structures play a role in dental eruption?
Introduction: The gubernacular cord is an original structure of the dental lamina, which undergoes apoptosis and their remnants were organized in the form of epithelial islets and strands that lined up, leaving the...
Association of two techniques of frontal sinus radiographic analysis for human identification
Introduction: The analysis of images with human identification purpose is a routine activity in the departments of forensic medicine,especially when is necessary to identify burned bodies, skeletal remains or corpses in...