Generation of recombinant bioluminescent Escherichia coli for quantitative determination of bacterial adhesion
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2015, Vol 28, Issue 4
Abstract
Bacterial adhesion to urinary catheter was evaluated by measuring the light emitted from a recombinant bioluminescent glycocalyx producer Escherichia coli strain. Generation of the bioluminescent strain was carried out by transforming the bacterial cells with pUCP18-GFP plasmid that contains a green fluorescence gene. Light emission measurement was closely correlated with the number of the adherent cells, giving a detectable signal from 1.2 X 102 cells. The efficiency of this assay was confirmed by testing the antiadherent effect of subinhibitory concentrations of ciprofloxacin with the aid of a model for in-vitro catheter colonization. There was no significant difference in the percentage reduction of adherent cells obtained by both light emission measurement and viable cell count techniques.
Authors and Affiliations
Khalid Abdul Rahman AlLuhaybi , Ghadah Yazeed Alghaith , Nayera Ahmed Moneib , Mahmoud Abdul Magead Yassien
Keywords
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