HPLC METHOD DEVELOPMENT AND VALIDATION: AN OVERVIEW
Journal Title: Int J of Preclin & Pharm Res - Year 2014, Vol 4, Issue 4
Abstract
ABSTRACT High performance liquid chromatography (HPLC) is an analytical technique which is proficient to separate, detect and quantify various drugs and its related degradants. An appropriate mobile phase, column, column temperature, wavelength and gradient must be found that affords suitable compatibility and stability of drug as well as degradants and impurities. This review covers the importance of RP-HPLC in analytical method development and their strategies along with brief knowledge of critical chromatographic parameters need to be optimized for an efficient method development. Keywords: Method validation, Method development, High PressureLiquid Chromatography (HPLC).
Authors and Affiliations
G. Santhosh*, G. Nagasowjanya, A. Ajitha, Y. Uma Maheswara Rao
Keywords
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