Impact of anthelmintic efficacy of Calotropis procera on tegumental enzymes of the trematode, Gastrothylax indicus
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2014, Vol 4, Issue 8
Abstract
The trematode parasite, Gastrothylax indicus was exposed to ethanolic and aqueous flower extract of Calotropis procera to evaluate the anthelmintic efficacy of the plant. The parasites were incubated in 6.25, 12.5, 25, 50 mg ethanolic and aqueous extracts per ml of PBS at a temp of 37± 1ºC. Mortality was observed at 0.5± 0.05h and 0.75± 0.10h for ethanolic and aqueous extracts respectively for the parasite at the highest test concentration of the plant extract. The commercial anthelmintic albendazole was tested for various concentrations ranged from 20-80 μg/ml and mortality was observed instantly (0.08±0.01h) at the concentration of 80 μg/ml. To further investigate the efficacy of plant extract, vital tegumental enzymes of the parasite viz. Alkaline phosphatase (ALP), Acid phosphatase(ACP), Adenosine triphosphatase (ATPase) and Glucose- 6-phosphatase (G-6-pase) was found to be suppressed by 43.890, 30.287,18.970 and 22.842% by ethanolic extract and 62.710, 19.780, 57.554 and 10.035% by aqueous extract whereas albendazole inhibited 41.617, 25.650, 64.797 and 26.611% respectively. Enzyme kinetic studies showed inhibition to be non-competitive in case of ACP with both the extracts and albendazole whereas for ALP it was found to be non- competitive with ethanolic and mixed type with aqueous extract. Albendazole showed competitive inhibition in case of ALP
Authors and Affiliations
Rama Aggarwal , Upma Bagai
Keywords
Related Articles
- EP ID EP88808
- DOI -
- Views 101
- Downloads 0
Sulphasalazine Induced Toxic Epidermal Necrolysis A Case Report
Toxic Epidermal Necrolysis (TEN) is a rare and life threatening mucocutaneous reaction characterized by extensive necrosis and detachment of epidermis. The Worldwide incidence of TEN is 0.9 to 1.4 per million popula...
Effect of Breath’In On Oxidative Pulmonary Damage and Fibrosis in A Rodent Model
Aim: The present study evaluated the protective effects of Breath’In, on oxidative damage and fibrosis in the lungs of rats. Method: Pulmonary damage and fibrosis was induced in female Sprague Dawley rats using CCl4 (3 m...
Extraction and Purification of Yellowfin Tuna Fishbone Flour as an Ingredient of Future Traditional Medicine
ABSTRACT: Yellowfintunafishbone flour contains high macro and micro minerals from its bone which can be used as an alternative ingredients to supply minerals for those who are allergic to dairy products. High quality ext...
Theoretical Spectral investigation of a bioactive Benzenoid Isolated from Mimusops elengi
The bioactive aromatic aldehyde, vanillin (3-methoxy 4-hydroxy benzaldehyde) is isolated from stem bark of the medicinal plant Mimusops elengi for the first time. Elucidation of structure is achieved with the hel...
Pattern of Acute Poisoning Presenting at a Tertiary Care Hospital
Abstract: Acute poisoningis a medical emergency. It is important to know the nature, severity and outcome of acute poisoning cases in order to take up appropriate planning, prevention and management techniques. This stud...