Investigation of the Effects of Temperature and Starvation Stresses on Microspore Embryogenesis in Two Tetraploid Roses (Rosa Hybrida L.)
Journal Title: Scientia Agriculturae - Year 2016, Vol 14, Issue 2
Abstract
In this research work, the isolated microspore culture system in two rose cultivars i.e. ‘Apollo’ and ‘Amarosa’ was investigated. Important factors including isolation media (AB, B), AT3 induction medium with different carbohydrate sources (sucrose, maltose and glucose) and amino acids source (lactalbumin hydrolysate) were studied. Stress is an important factor that could de-differentiate and regenerate microspores to a whole plant. Carbon starvation and temperature (heat and cold) treatments as two important stresses alone or in combination with each other for various periods were evaluated on the induction of sporophytic divisions. A mixture of different developmental stages of microspores was used to initiate the cultures but the majority of them were at late uni-cellular stage determined by DAPI staining. For eliminating bacterial or fungal contaminants, buds were surface-sterilized by immersion in 70% ethanol for 15, 30, 60 Sec. and 3.5% sodium hypochlorite solution for 5, 10, 15 min prior to microspore isolation. The best result was observed when microspores were sterilized with sodium hypochlorite (%3.5) for 10 minutes. Two isolation media did not show significant difference on the viability of microspores. Among induction media, in cv. ‘Amarosa’, the highest viability was observed in AT3 induction medium supplemented by glucose. Induction media in ‘Apollo’ cultivar didn’t show significant difference in microspores viability. Combination of starvation (B medium) and cold (4°C) treatment for 3 days induced embryo formation in cv. ‘Amarosa’. Present investigation for the first time reports a protocol for embryogenesis induction in rose (Rosa hybrida L.) microspores.
Authors and Affiliations
Maryam Dehestani-Ardakani, Mehran E. Shariatpanahi, Mohsen Kafi
Keywords
Related Articles
- EP ID EP133495
- DOI 10.15192/PSCP.SA.2016.14.2.220227
- Views 72
- Downloads 0
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