Novel RP-HPLC Method for Simultanious Determination of Sitagliptin and Simvastation in Bulk and Tablet Dosage Form
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2015, Vol 5, Issue 8
Abstract
A simple, rapid, accurate, precise and novel high-performance liquid chromatographic method for simultaneous analysis of Sitagliptin (SITA) and Simvastation (SIMV) in pharmaceutical dosage form has been developed and validated. The chromatographic separation was accomplished on Welchrom RP-C18 Column (250 mm X 4.6 mm; 5µm), Shimadzu LC-20AT Prominence Liquid Chromatograph and with a mixture of 10 mM Phosphate buffer: acetonitrile and methanol in the range of (45:35:20 v/v/v). The flow rate was fixed at 1mL/minute and the analysis was performed using Shimadzu SPD-20A Prominence UV-detection was performed at 255 nm. The SITA and SIMV were separated within seven minutes. The retention time for SITA and SIMV was found to be 3.352 minutes and 5.402 minutes respectively. The calibration plots were linear over the concentration range of 10-50 µg/ml for SITA (r2 = 0.9998) and 4-20 µg/ml for SIMV (r2 = 0.9999). There was no interference due to commonly used excipients. The relative standard deviation for inter-day precision was lower than 2.0 % which obviously indicates that the present method was said to be highly precise. Regarding accuracy of the developed method the % RSD were also found less than 2 % which shows the method is completely accurate. The method was very sensitive with regard to LOD 0.681 µg/ml, 0.116 µg/ml and LOQ 2.250 µg/ml, 0.384 µg/ml respectively. The mean assay values for SITA and SIMV were determined in tablet dosage form were found to be within limits. The developed RP HPLC method was found to be simple, rapid, sensitiv
Authors and Affiliations
P. Ravisankar*1 , SK. Hassain1 , Shaik Mohammed Neeha1
Keywords
Related Articles
- EP ID EP148411
- DOI -
- Views 99
- Downloads 0
Sensitivity and specificity of 99m Tc-UBI29-41 and 67 Ga-Citrate scintigraphy imaging to discriminate infection lesion induced by Staphylococcus aureus and sterile inflammation lesion induced by Carrageenan in foot’s rat
This study was launched to evaluate the efficacy and efficiency of 99m Tc-UBI29-41scintigraphy imaging to visualize the infection foci induced by staphylococcus aureus and inflammation lesions induced by carra...
[b]GALLIC ACID PRODUCTION AND TANNASE ACTIVITY OF PENICILLIUM PURPUROGENUM STOLL EMPLOYING AGROBASED WASTES THROUGH SOLID STATE FERMENTATION : INFLUENCE OF CARBON AND NITROGEN SOURCES[/b]
Chemical constituents, nutritional, pharmcological and therapeutic importance of Juglans regia- A review
The phytochemical analysis of the bark of Juglans regia showed that it contained reducing sugars, alkaloid, tannins, phenols and saponins. Preliminary phytochemical test of the leaves of Juglans regia showed the presence...
FIBROLIPOMATOUS HAMARTOMA OF ULNAR NERVE: A RARE CASE REPORT.
Nervous fibrolipomatous hamartoma is said to be a rare tumor-like condition involving the peripheral nerves,in which the epineurium and perineurium are enlarged and distorted by excess of fatty and fibrous tissue s...
Effect of Nonsteriodal Anti-Inflammatory Drugs on Orthodontic Tooth Movement – Review
Orthodontic tooth movement is a biological response towards mechanical force resulting in remodeling of the periodontal structure allowing for tooth movement. Periodontal remodelling is a complex process regu...