Novel RP-HPLC Method for Simultanious Determination of Sitagliptin and Simvastation in Bulk and Tablet Dosage Form
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2015, Vol 5, Issue 8
Abstract
A simple, rapid, accurate, precise and novel high-performance liquid chromatographic method for simultaneous analysis of Sitagliptin (SITA) and Simvastation (SIMV) in pharmaceutical dosage form has been developed and validated. The chromatographic separation was accomplished on Welchrom RP-C18 Column (250 mm X 4.6 mm; 5µm), Shimadzu LC-20AT Prominence Liquid Chromatograph and with a mixture of 10 mM Phosphate buffer: acetonitrile and methanol in the range of (45:35:20 v/v/v). The flow rate was fixed at 1mL/minute and the analysis was performed using Shimadzu SPD-20A Prominence UV-detection was performed at 255 nm. The SITA and SIMV were separated within seven minutes. The retention time for SITA and SIMV was found to be 3.352 minutes and 5.402 minutes respectively. The calibration plots were linear over the concentration range of 10-50 µg/ml for SITA (r2 = 0.9998) and 4-20 µg/ml for SIMV (r2 = 0.9999). There was no interference due to commonly used excipients. The relative standard deviation for inter-day precision was lower than 2.0 % which obviously indicates that the present method was said to be highly precise. Regarding accuracy of the developed method the % RSD were also found less than 2 % which shows the method is completely accurate. The method was very sensitive with regard to LOD 0.681 µg/ml, 0.116 µg/ml and LOQ 2.250 µg/ml, 0.384 µg/ml respectively. The mean assay values for SITA and SIMV were determined in tablet dosage form were found to be within limits. The developed RP HPLC method was found to be simple, rapid, sensitiv
Authors and Affiliations
P. Ravisankar*1 , SK. Hassain1 , Shaik Mohammed Neeha1
Evaluation of Wound Healing Activity of Methanolic Extract of Tonna dolium (L.1758)
Marine invertebrates offer a rich source of potential drugs with excellent biological activities. The present study was aimed at investigating the wound healing effect of methanolic extract of Tonna dolium (L.1758) using...
Phytochemical Screening and In-vitro Antibacterial Activity of Mangifera indica (Mango) Kernel on Aeromonas caviae
[b] OBJECTIVE: [/b] To determine the phytoconstituents of Mangifera indica kernel and its antibacterial activity against Aeromonas caviae. [b]METHODS: [/b]Methanol extract of mango kernel was used to identi...
Substance Abuse among Adolescents: 2. Prevalence and Patterns of Cigarette smoking among senior secondary school students in Abraka, Delta State, Nigeria
: Cigarette smoking is an important health hazard and a major preventable cause of morbidity and mortality. More young people are getting involved in tobacco use in Nigeria. This is of great concern. This study...
[b] Evaluation of Antimicrobial Activity of Aqueous and Hydro-Alcoholic Curcuma Longa Extracts against Endodontic Pathogens[/b]
Chemical Composition, Free Radical Scavenging And Antifungal Activity of Zanthoxylum Leprieurii Essential Oils Against Epidermophyton Floccosum And Microsporum Gypseum, two Most Prevalent Cutaneous Mycosis
Background: These investigations were carried out to analyze the chemical composition, to evaluate the radical scavenging, and the anti-fungal activities of essential oils from leaves and fruits of Zanthoxylum leprieurii...