Production of itaconic acid by Ustilago maydis from agro wastes in solid state fermentation
Journal Title: Journal of BioScience and Biotechnology - Year 2014, Vol 3, Issue 2
Abstract
Itaconic acid (IA) is one of the hopeful substances within the cluster of organic acids. IA is used in artificial glass, bioactive compounds in pharmacy, medicine, agriculture, for the synthesis of fiber, resin, plastic, rubber, paints, surfactant, ion-exchange resins and lubricant. Most recurrently used microorganism for commercial production of IA is Aspergillus terreus. Some filamentous fungi belonging to Ustilaginales also produce IA. In the present work, an attempt was made to produce IA by Ustilago maydis employing Solid State Fermentation (SSF) from various agro wastes like ground nut shells, rice bran, rice husk, orange pulp, ground nut oil cake, orange pulp and sugarcane bagasse as carbon substrates, which were used after pretreatment. 10 g of each substrate was taken in a 500 ml conical flasks separately and supplemented with 20 mL nutrient solution containing glucose, at pH 3. One milliliter inoculum containing 1×107 spores was added and moisture was maintained at 60%. After incubation at 32°C for 5 days, the acid production was estimated by spectrophotometric method and by HPLC analysis. Interestingly, the yield of itaconic acid was promising with all the above substrates, where orange pulp, sugarcane bagasse and rice bran supported higher yields.
Authors and Affiliations
Mokula Rafi, M. Hanumanthu, D. Rao, Kadiyala Venkateswarlu
Keywords
Related Articles
- EP ID EP152975
- DOI -
- Views 73
- Downloads 0
Use of in vitro gas production technique to evaluate the effects of microwave irradiation on sorghum (Sorghum bicolor) and wheat (Triticum sp.) nutritive values and fermentation characteristics
Effects of microwave irradiation (900 W) for 3, 5 and 7 min on the nutritive value of sorghum and wheat grains were evaluated by in vitro gas production technique. Gas volume was recorded at 2, 4, 6, 8, 12, 16, 24, 36, 4...
GC-MS based metabolite profiling of five Bulgarian Fumaria species
The aim of this study was profiling of primary metabolites of five Bulgarian Fumaria species (F. officinalis L., F. thuretii Boiss., F. kralikii Jord., F. rostellata Knaf. and F. schrammii Velen.) by GC-MS analyses...
Attitudes of university students of biology towards bees and their protection
By the number of species, insects are the most numerous group of invertebrates. They play an important role in nature, in ecosystems. Especially important are insect pollinators that provide survival for plants and other...
Leuconostoc mesenteroides NRRL B-1149 as probiotic and its dextran with anticancer properties
Leuconostoc mesenteroides NRRL B-1149 was evaluated for its probiotic properties. It displayed in vitro cell surface traits in terms of hydrophobicity, autoaggregation property and cell adhesive capacity on HeLa, m...
Antioxidant activity and fructan content in root extracts from elecampane (Inula helenium L.)
The purpose of the present study was to investigate the antioxidant activity and fructans content in ethanol and water extracts of roots of elecampane (Inula helenium L.), a Bulgarian medicinal plant. The extractio...