Simultaneous Determination of Cefotaxime Sodium and Paracetamol by LC-MS
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2014, Vol 4, Issue 2
Abstract
A rapid and sensitive liquid chromatography-mass spectrometry (LC-MS) method has been developed and validated for simultaneous determination of cefotaxime sodium and paracetamol. Sulfamethoxazole was used as the internal standard. A phenomenex Luna C18 column provided chromatographic separation of the analyte which was followed by detection with mass spectrometry. The mass transition ion-pair was followed as m/z 152.1 for paracetamol and 478.1 m/z for cefotaxime sodium. The mass transition of internal standard sulfamethoxazole was found at 254.0 m/z. The method involves simple isocratic chromatography conditions with mobile phase acetonitrile:20mM ammonium acetate buffer in the ratio of 40:60 and mass spectrometric detection that enables detection at ppm levels. The retention times were 3.477 and 5.601 min for Cefotaxime sodium and paracetamol respectively. The proposed method has been validated with linear range of 1-40 ppm for both the drugs. The precision values are ≤ 2 %. The accuracy of the method was found to be 98-102 %.
Authors and Affiliations
,C H Latha Saranya , 2,J C Thejaswini , 3,B M Gurupadayya , 4,B Y K Sruthi
Keywords
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