Simultaneous Determination of Cefotaxime Sodium and Paracetamol by LC-MS
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2014, Vol 4, Issue 2
Abstract
A rapid and sensitive liquid chromatography-mass spectrometry (LC-MS) method has been developed and validated for simultaneous determination of cefotaxime sodium and paracetamol. Sulfamethoxazole was used as the internal standard. A phenomenex Luna C18 column provided chromatographic separation of the analyte which was followed by detection with mass spectrometry. The mass transition ion-pair was followed as m/z 152.1 for paracetamol and 478.1 m/z for cefotaxime sodium. The mass transition of internal standard sulfamethoxazole was found at 254.0 m/z. The method involves simple isocratic chromatography conditions with mobile phase acetonitrile:20mM ammonium acetate buffer in the ratio of 40:60 and mass spectrometric detection that enables detection at ppm levels. The retention times were 3.477 and 5.601 min for Cefotaxime sodium and paracetamol respectively. The proposed method has been validated with linear range of 1-40 ppm for both the drugs. The precision values are ≤ 2 %. The accuracy of the method was found to be 98-102 %.
Authors and Affiliations
,C H Latha Saranya , 2,J C Thejaswini , 3,B M Gurupadayya , 4,B Y K Sruthi
Keywords
Related Articles
- EP ID EP120963
- DOI -
- Views 103
- Downloads 0
A high frequency microcloning protocol for subsequent cryopreservation in Kaempferia galanga L.: An endangered, over exploited medicinal plant in pharmaceutics
: An efficient in vitro propagation protocol was standardized in K. galanga, wherein shoot cultures were raised from rhizome with axillary bud explants in Murashige and Skoog (MS) medium supplemented with differe...
Development And Optimization of Boswellia Serrata Self-Micro Emulsifying Formulation: An Ameliorative Effort Towards The Herbal Formulation
Abstract: Development of self-micro emulsifying drug delivery system (SMEDDS) of herbal extracts is challenging task per se, as the herbal extracts contains many active and non-active constituents with various physicoche...
Synthesis, Characterization, Biological Activity and DNA Cleavage Studies on Tetrazole Imine Base and Their Metal Complexes - An Experimental and Theoretical Approach
Novel Tetrazole imine and its binary Cu(II) and Ni(II) complexes were synthesized and characterized by employing spectro - analytical techniques viz; Mass, UV, IR, 1H NMR, elemental analyses, thermo-analytical me...
A case of allergy and food sensitivity: the nasunin, natural color of eggplant
llergies and food sensitivities can both be considered as "adverse reactions individualistic" to food. Are pathological and individual forms because they affect a few individuals in way rather serious; immediate&n...
Comparative Study of Hscrp in Chronic Kidney Disease
BACKGROUND: Chronic kidney disease (CKD) is a global threat to health mainly in developing countries be cause therapy is expensive and lifelong. over 1 million people worldwide are on dialysis or with a functi...