OBTAINING TRANSGENIC PLANTS BEARING REPORTER GENE UNDER CONTROL OF APX2 PROMOTER
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2014, Vol 6, Issue 2
Abstract
Ascorbate peroxidase (APX) represents an enzyme involved in plant cell protection against oxidative stress. In Arabidopsis thaliana, APX is encoded by a small multigenic family comprising 8 genes. The 8 isoforms of APX present in chloroplasts, peroxisomes and cytosol are differentially activated in response to abiotic stress. Especially, Apx2 is a typical stress-inducible gene, which is transcriptionally active only upon stressful conditions. In order to elucidate stress-dependent expression of Apx2 in different organs and tissues of A. thaliana promoter region of the gene was amplified by PCR and cloned. The promoter was fused with uidA reporter gene coding for β-glucuronidase and introduced into A. thaliana wild type plants applying Agrobacterium-mediated transformation. Transgenic plants were identified using treatment with herbicide BASTA. Presence of the recombinant construct in plants was confirmed by PCR. In total, 7 independent lineages bearing Pro-APX2:GUS fusion construct were isolated.
Authors and Affiliations
Т. О. РУСНАК, І. І. ПАНЧУК
Keywords
Related Articles
- EP ID EP246404
- DOI -
- Views 99
- Downloads 0
The impact of DON-1R on Microcystis sp. monocultures
The study is intended to investigate the impact of complex drug DON-1R on monocultures of Microcystis sp. Cyanobacteria of Microcystis aeruginosa (Kützing) Kützing та Microcystis pulverea (H.C.Wood) Forti are the dominan...
Complex selection in vitro of sugar beet (Beta vulgaris L.) for droughtproof and solismity
In recent years, the development of crop production is aimed at creating, capable of responding quickly to stressful effects and subsequent self-regulation of agro-ecosystems. One of the promising directions that make it...
Можливі механізми захисної дії альфа-кетоглутарату за впливу різних стресорів на плодову мушку drosophila melanogaster canton s
Альфа-кетолутарова кислота – важливий інтермедіат циклу Кребса та метаболізму амінокислот. В останні роки показана роль альфа-кетоглутарової кислоти як потужного природного детоксифікуючого агенту. У даній роботі дослідж...
Honey bee (Apis mellifera L.) colony losses in Ukraine after the winter of 2016-2017 within the international monitoring
Honey bee colony losses are considered to be a global problem nowadays. The international non-profit honey bee research association COLOSS has been investigating this issue since 2008 (Neumann & Carreck, 2010; van der Ze...
The intensity of free radical processes in the liver mitochondrial fraction under the conditions of toxic injury and correction by the 3,4-dihydropyrimidine-2-one ammonium derivative
The research deals with the determination of the superoxide-anion radical, TBA-active products and protein carbonyl derivatives content in the protein-deficient rat liver mitochondrial fraction under the conditions of ac...