The intensity of free radical processes in the liver mitochondrial fraction under the conditions of toxic injury and correction by the 3,4-dihydropyrimidine-2-one ammonium derivative
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2017, Vol 9, Issue 2
Abstract
The research deals with the determination of the superoxide-anion radical, TBA-active products and protein carbonyl derivatives content in the protein-deficient rat liver mitochondrial fraction under the conditions of acetaminophen-induced hepatitis and correction by the 3,4-dihydropyrimidine-2-one ammonium derivative. The researches were conducted on white rats of 90-100 g body mass aged 2-2.5 months. There were used rats, which according to the experimental model were separated into the following groups: I – animals receiving full-value semi-synthetic ration (C); II – animals receiving low-protein ration (LPR); III – animals with acetaminophen-induced liver injury receiving complete ration (H); IV – animals with acetaminophen-induced liver injury that were previously maintained on semi-synthetic low-protein ration (LPR+H); V-XІІ – animals, which were introduced with the examined 3,4-dihydropyrimidine-2-one ammonium derivative at concentrations of 5; 10; 15; 20; 25; 30; 40; 50 mg/kg of the body weight correspondingly. The examined compound concentrations were introduced orally in 2 hours after acetaminophen exposure during 2 days according to GLP recommendation. The most pronounced antioxidant effect at concentration of 15 mg/kg of the 3,4-dihydropyrimidine-2-one ammonium derivative was established. It is observed that the introducing of the examined compound in the specified concentration leads to the maximal decrease of superoxide-anion radical, TBA-active products and protein carbonyl derivatives content in the rat liver mitochondrial fraction with toxic injury to control values. The established patterns are confirmed by a correlation analysis, which was shown the existence of a close correlation between the concentration of test compound and superoxide-anion radical content. The 3,4-dihydropyrimidine-2-one ammonium derivative is a promising compound for the creation on its basis of an antioxidant agent which is capable to effectively suppression of the intensity of free radical processes in the liver mitochondria under the conditions of toxic injury.
Authors and Affiliations
Oksana Voloshchuk, Galina Kopylchuk, Yuliia Mishyna
Keywords
Related Articles
- EP ID EP262296
- DOI 10.31861/biosystems2017.02.197
- Views 100
- Downloads 0
Antioxidant system activity in liver and guerin’s carcinoma of rats under conditions of ω-3 polyunsaturated fatty acids administration
The effect of ω-3 polyunsaturated fatty acids (PUFAs) supplementation on superoxide dismutase аnd catalase activities as well as the level of alpha-tocopherol and ascorbate were monitored in mitochondrial fraction of rat...
The qualities of theta-activity of electroencephalogram of a brain of people with different power of neural processes during the verbal and non-verbal activity
The qualities of the theta-activity of an electroencephalogram (EEG) of subjects’ cerebral cortex with differing power of neural processes of the subjects performed verbal and non-verbal cognitive tasks were investigated...
ACTIVITY OF THE ENZYMES OF THE CARBOHYDRATE METABOLISM IN MUSCLE TISSUE OF RATS WITH EXPERIMENTAL DIABETES FOR THE COMPLEX ACTION OF MAGNESIUM AND CHROMIUN CITRATES
The aim of our research was to investigate the complex action of magnesium citrate (in the dose of 250 mg Mg2+/kg b.w.) and chromium citrate (in doses of 25 μg Cr2+/kg b.w. and 10 μg Cr2+/kg b.w.) on the functional state...
Honey bee (Apis mellifera L.) colony losses in Ukraine after the winter of 2016-2017 within the international monitoring
Honey bee colony losses are considered to be a global problem nowadays. The international non-profit honey bee research association COLOSS has been investigating this issue since 2008 (Neumann & Carreck, 2010; van der Ze...
Залежність між фізико-хімічними характеристиками жолудів дуба черешчатого (Guercus robur L.) в популяціях українського степу і коефіціентом їх форми
Одним з перспективних напрямків господарської діяльності індустріально розвинених країн є забезпечення продовольчої безпеки і формування раціонального і ефективного використання альтернативних джерел поживних компонентів...