Occurrence of Escherichia coli harbouring stx genes in popiah, a Malaysian street food
Journal Title: Food Research - Year 2017, Vol 1, Issue 1
Abstract
Irrespective of its health effects, street foods are very popular with the consumers. The main purpose of this research was to study the biosafety of Escherichia coli in popiah, a Malaysian street food sold at a roadside food stall and a restaurant in Sri Serdang, Selangor, Malaysia, using the combination of the most probable number (MPN)-Polymerase Chain Reaction (PCR) assay-plating on Eosin Methylene Blue (EMB) agar methods. Using these biomolecular methods, E. coli was detected in 12/15 (80%) and 11/15 (73%) of the collected samples from the roadside food stall and the restaurant respectively. The incidence of stx virulence-associated genes was detected in 1/15 (7%) among the E. coli isolated from samples taken from the roadside food stall while the E. coli isolated from the restaurant was 3/15 (20%). The density of E. coli ranged from <3 to >1100 MPN/g and the density of E. coli positive with stx genes was <3 to 53 MPN /g in samples from both the roadside food stall and the restaurant. The presence of the stx-positive E. coli in popiah are significant to risk assessments of food and epidemiological studies. Therefore, from the information obtained in this study, it is obvious that the screening for STEC markers in food samples would be useful for food safety survey.
Authors and Affiliations
Elexson N. , Nik Yuhanis, F. N. , Malcolm T. T. H. , C. Y. New, W. S. Chang, Ubong A. , C. H. Kuan, Y. Y. Loo, T. Y. Thung, Son R.
Keywords
Related Articles
- EP ID EP289046
- DOI 10.26656/fr.2017.1.008
- Views 244
- Downloads 0
Physical and functional properties of Indian squid (Loligo duvauceli) and cuttlefish (Sepia latimanus) ink powder
This study aimed to compare the physical and functional properties of Indian squid (Loligo duvauceli) and cuttlefish (Sepia latimanus) ink powders. Indian squid and cuttlefish are common major sources of seafood products...
Tempeh consumption enhanced beneficial bacteria in the human gut
It has been known that human gut microbiota modulates host health and the composition is affected by lifestyle, including the availability of dietary fiber. Tempeh, a kind of traditional fermented food from Indonesia, is...
Effect of sunlight on the physicochemical properties of refined bleached and deodorized palm olein
In this study, the effect of sunlight on the quality of palm olein was assessed. Palm olein samples were subjected to sunlight (34-40°C) and ambient storage (~24°C) for 90 days. Oil samples were collected at 30 days inte...
Antioxidant and antimicrobial activities of squid ink powder
Economic development in Malaysia has led to increasing quantity and complexity of generated waste or by-product. The main objective of this study is to investigate the antioxidant and antimicrobial activities of squid in...
Improving meatball quality using different varieties of rice bran as natural antioxidant
Meatballs are very popular in every social class in many developing countries including Indonesia and are produced both under the cottage or commercial meat processing industries. Rice bran contains antioxidants and ther...