One-Step Nucleic Acid Amplification – a new method for the detection of metastases of solid tumours of the lymph nodes
Journal Title: Medycyna Ogólna i Nauki o Zdrowiu - Year 2015, Vol 21, Issue 2
Abstract
Introduction and purpose. None of the applied methods of lymph node assessment is perfect. The need to wait a long time for the results is the disadvantage of postoperative histopathological examination. In the case of a positive outcome of the post-operative histopathology of sentinel lymph node it is necessary to perform a second surgery. Some metastases smaller than 2 mm can be omitted in routine histopathology. In turn, frozen section examination, with relatively high specificity, has lower sensitivity than postoperative histopathology. Molecular assays, using PCR techniques, with high sensitivity, have lower specificity. In addition, except for real-time PCR, all other techniques are not suitable for use as an intraoperative examination due to the long waiting time for the result. OSNA is one of the latest methods of lymph node assessment in solid tumours. The article summarizes world reports on the application of this technique in the evaluation of lymph nodes in patients with solid tumours. Summary of the state of knowledge. The OSNA test is based on mRNA for cytokeratin 19(CK19) amplification. At present, this test is often performed in the search for lymph nodes metastases in patients with breast cancer. Intraoperative evaluation of sentinel lymph nodes in breast cancer, using the OSNA method, allows deciding whether or not to perform lymphadenectomy. In other cancers, the use of the OSNA method has been evaluated in numerous clinical trials. Summary. The OSNA method could become a routine technique for the assessment of lymph nodes. It has a relatively high sensitivity and allows evaluation of lymph nodes in 30 minutes, which enables its use as an intraoperative examination.
Authors and Affiliations
Krzysztof Kaczka, Anna Grzegory, Lech Pomorski
The course of pregnancies and deliveries in a patient with a bihornal bicervical uterus and partially-septated vagina
The report presents the course of pregnancies and deliveries in a patient with uterine and cervical malformation, i.e. a bihornal bicervical uterus and partially-septated vagina.Delivery 1: terminated by Caesarean secti...
Nebulization therapy in children with cystic fibrosis – opinions of the children and their parents
Introduction: Nebulization is considered a very good and effective method for inhaled drug delivery. Despite some disadvantages, its effectiveness, accessibility and ease of use has resulted in it attaining an establishe...
Evaluation of the level of knowledge of selected cardiovascular risk factors among adolescents attending secondary schools in Warsaw
Objective: Numerous data from literature show that the most efficient method of control of the epidemic of cardiovascular diseases is control of the risk factors within the primary prevention, as well as health-promotin...
Dzieci jako sprawcy i ofiary wypadków komunikacyjnych w Polsce w latach 2008–2015
[b]Wprowadzenie.[/b] Wypadki komunikacyjne z udziałem dzieci są istotnym zagrożeniem prowadzącym do urazów będących powodem niepełnosprawności, a także stanowią przyczynę zgonów możliwych do uniknięcia.[b]Cel pracy.[/b]...
Multi-level care of a child with cerebral palsy
Introduction and objective: The purpose of this paper is to present the needs of a child with infantile cerebral palsy, based on our own experiences, and to present the views of co-authors – a paediatrician and a physiot...