One-Step Nucleic Acid Amplification – a new method for the detection of metastases of solid tumours of the lymph nodes

Journal Title: Medycyna Ogólna i Nauki o Zdrowiu - Year 2015, Vol 21, Issue 2


Introduction and purpose. None of the applied methods of lymph node assessment is perfect. The need to wait a long time for the results is the disadvantage of postoperative histopathological examination. In the case of a positive outcome of the post-operative histopathology of sentinel lymph node it is necessary to perform a second surgery. Some metastases smaller than 2 mm can be omitted in routine histopathology. In turn, frozen section examination, with relatively high specificity, has lower sensitivity than postoperative histopathology. Molecular assays, using PCR techniques, with high sensitivity, have lower specificity. In addition, except for real-time PCR, all other techniques are not suitable for use as an intraoperative examination due to the long waiting time for the result. OSNA is one of the latest methods of lymph node assessment in solid tumours. The article summarizes world reports on the application of this technique in the evaluation of lymph nodes in patients with solid tumours. Summary of the state of knowledge. The OSNA test is based on mRNA for cytokeratin 19(CK19) amplification. At present, this test is often performed in the search for lymph nodes metastases in patients with breast cancer. Intraoperative evaluation of sentinel lymph nodes in breast cancer, using the OSNA method, allows deciding whether or not to perform lymphadenectomy. In other cancers, the use of the OSNA method has been evaluated in numerous clinical trials. Summary. The OSNA method could become a routine technique for the assessment of lymph nodes. It has a relatively high sensitivity and allows evaluation of lymph nodes in 30 minutes, which enables its use as an intraoperative examination.

Authors and Affiliations

Krzysztof Kaczka, Anna Grzegory, Lech Pomorski


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  • EP ID EP58466
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How To Cite

Krzysztof Kaczka, Anna Grzegory, Lech Pomorski (2015). One-Step Nucleic Acid Amplification – a new method for the detection of metastases of solid tumours of the lymph nodes. Medycyna Ogólna i Nauki o Zdrowiu, 21(2), 120-124.