OPTIMIZATION OF PROCEDURE OF SEPARATE BIOSENSOR DETECTION OF AFLATOXINS AND PESTICIDES
Journal Title: Сенсорна електроніка і мікросистемні технології - Year 2016, Vol 13, Issue 4
Abstract
A procedure of separate biosensor analysis of the multicomponent sample with aflatoxins and pesticides has been developed and optimized. Biosensor determination of aflatoxins and pesticides was performed using enzyme inhibition analysis. For creation of bioselective element we used enzyme acetylcholinesterase which is co-immobilized with bovine serum albumin on the surface of potentiometric transducer by glutaraldehyde covalent crosslinking. As transducers were pH-sensitive field effect transistors. The concentration of acetylcholine chloride as a substrate for subsequent inhibition analysis was fit; optimal time of inhibition by toxins solution was determinate together with concentration of reactivator (pyridine-2-aldoxymmethyliodyd) and time of enzyme reactivation after inhibition. A synergism between trichlorfon and aflatoxin B1 in inhibition of immobilized on a surface pH-sensitive field-effect transistors acetylcholinesterase was investigated. The proposed procedure allows selective determination the composition of the multicomponent sample with aflatoxins and pesticides
Authors and Affiliations
М. Yu. Коrobko, К. V. Stepurska, О. О. Soldatkin, V. N. Arkhypova, S. V. Dzyadevych
Keywords
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- EP ID EP397136
- DOI 10.18524/1815-7459.2016.4.86653
- Views 95
- Downloads 0
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