PartialPurificationAndCharacterizationOfPolyphenolOxi daseFromWild Pears (Pyrus communis)
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2013, Vol 4, Issue 11
Abstract
precipitation,dialysisandionexchangechromatography.Ofthesubstratestested, pyrogallol wasthebettersubstrateforPPOwithaKm value of8.7mM at pH 5 and catechol is better with Km value 4.7 mM at pH 7.Theenzymeshowed high activity overabroad pHrange of4 – 8 so theoptimum pHforPPOactivity was foundtobe5 and 7. Theoptimal temperature forenzymeactivity wasfound tobe 45°C in presence of pyrogallol but 55°Cwith catechol as substrate at pH 7.Km value for wild pear PPO is calculated 9.5 and 4.7 mM for catechol and 8.7 and 5.1 mM for pyrogallol at pHs 5 and 7 respectively.Ascanbeseen,affinity ofPPOsforvarious substrates varies widely.Theenzymeshowedabroad activityoverabroadpHand temperaturerange. Thethermalinactivation studies showedthatthe enzyme isheat resistant.The enzymeshowed thehighest activity toward pyrogallol and noactivity towardtyrosine. Of the inhibitors tested, the mostpotent inhibitors was sodium kojic acid.
Authors and Affiliations
Shahryar saeidian *| Assistan professor of Biochemistry, Department of Biology, Payame Noor University, Iran, saeedyan@pnu.ac.ir, Afsane khosravi| Steudent of Master Science of Azad University
Keywords
Related Articles
- EP ID EP5769
- DOI -
- Views 292
- Downloads 8
Impact of particle attributes on granular material
The characteristics of its particles have an important effect on the mechanical behavior of granular materials. Granular materials are treated as a continuum and its particulate nature is not explicitly considered. This...
Design and Simulation of Z-Source Inverter for Brushless DC Motor Drive
This paper presents a Z-source inverter for controlling the speed and reducing the torque ripple of a brushless DC motor. This inverter uses a unique impedance network, coupled between a power source and the inverter cir...
A qualitative study to developingtheteacher’sprofessional standards
This study aims to design an indigenous model of professional standards that measure instructional and professional performance of high school teachers, particularly in the teaching - learning environments. The research...
Study on Effect of Water on Stability or Instability of the Earth Slopes
Water is the origin of the life, but the existence of water in earth slopes can be lead to irreparable damages. Water due to the fluid nature can be easily moved in the soil pores and due to reducing the effective stress...
Non-Retroactive Challenges of the Criminal Law in the Procedure of International Criminal Court
The principle of non-retroactivity rule is foundation of the law. So that this principle in the private law, public law and criminal law, even in international documents has a particular importance as a means of proving...