PCR in tuberculosis: An extremely useful tool?
Journal Title: IP International Journal of Medical Microbiology and Tropical Diseases - Year 2018, Vol 4, Issue 2
Abstract
Introduction Tuberculosis is a global air borne infectious disease with significant increase in incidence and prevalence Conventional methods take longer time for diagnosis and drug susceptibility with less sensitivity and specificity So rapid diagnosis and detection of multi extensive and total drug resistant bacilli are of up most importance for therapeutic purposeMaterials and Methods It was a retrospective study in tertiary care All clinical samples suspected for tuberculosis received by laboratory were included in the study The samples were processed for microscopy by ZN stain culture by LJ media and RTPCRResults Total 566 sample were tested out of 177 31 were positive for tuberculosis 62 samples were PCR positive 51 were culture positive and 49 were smear positive The most common sample received with maximum positivity rate was pleural fluid 34 Male predominance was seen with young adults as the predominant group affectedConclusion The present study shows good specificity of PCR with sensitivity of 50 It detects 50 times more cases in relation to conventional results where they fail to produce correct results More such comparative studies are needed to establish importance of PCR in early diagnosis and treatmentKeywords Mycobacteria RTPCR Diagnosis Detection Infection
Authors and Affiliations
Manali Shah, Ullas Bhabhor, Yogita Mistry, Summaiya Mullan
Keywords
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- EP ID EP485842
- DOI 10.18231/2581-4761.2018.0013
- Views 78
- Downloads 0
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