Bacterial spectrum in surgical wound infection, its susceptibility pattern and biofilm formation among isolates
Journal Title: IP International Journal of Medical Microbiology and Tropical Diseases - Year 2018, Vol 4, Issue 2
Abstract
Topic Bacterial spectrum in surgical wound infection its susceptibility pattern and biofilm formation among isolates Aim To identify the spectrum of bacterias associated with wound infection and their antimicrobial susceptibility pattern and biofilm formationIntroduction Chronic wounds are severe worldwide problem Wounds are considered chronic when healing fails to proceed normally and anatomic and functional integrity of the skin is not achieved in approximately 1 month Chronic wound include diabetic foot ulcer pressure or decubitus ulcer venous leg ulcer and nonhealing surgical site infection1Annual incidence is of diabetic ulcer in India in a population based study is 10 to 41 1 Biofilm formation is widespread in chronic wounds The biofilm phenotype gives rise to drug resistant strains resulting in treatment failureMaterials and Methods A prospective study was done at a tertiary care Hospital in North Karnataka for a period of 1 year from February 2017 to January 2018 A total of 241 samples in an age group of 20 to 70 years with history of wound infection attending surgery department were collected with a sterile cotton swab and processed as per CLSI standards2 Detection of biofilm formation by modified congo red agar method tissue culture plate method and tube assay methodResults A total of 241 pus samples were collected 6556 158 showed bacterial growth and 3443 83 showed no growth The study group comprised 139 male and 102 female patients in the ratio of 1391 Age range was 2070 years Maximum samples were in the age group of 2130 years 4493 71 of isolates were obtained Gram positive organisms and 5506 87 were Gram negative Escherichia coli was the predominant isolated organism 1618 39 Followed by staphylococcus aureus 954 23 76 out of 158 sample showed biofilm formation 4801 Staphylococcus aureus was dominant biofilm former 8157 62 of biofilm producers were multiple drugs resistantConclusion Detection of biofilm formation is an easy and cost effective test that can be performed routinely in the lab Biofilm will help surgeon to effectively manage these infections riding more aggressive source control and appropriate antibiotics resulting in decrease mortality and morbidity of patientsReferences Reiber GE Epidemiology of foot ulcers and amputations in the diabetic foot In Bowker JH Pfeifer MA editors Levin and OrsquoNealrsquos the diabetic foot 6th ed St Louis Mosby 2001p 1332Keywords Surgical wound infection Biofilm Antimicrobial resistance
Authors and Affiliations
Siddesh B. Sirwar, Nazneen Fatima
Keywords
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- EP ID EP485843
- DOI 10.18231/2581-4761.2018.0014
- Views 73
- Downloads 0
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