Phagocytosis and sockets forming activity of neutrophils in the latent course of equine infectious anaemia
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2017, Vol 30, Issue
Abstract
Introduction. One of the important problems in horse breeding is infectious anemia, which is constantly registered in many countries. Pathogen is a retrovirus, morphologically similar to bovine leukemia virus. Basic mechanisms of pathological process in organism were caused by immunological disorders. Researches indicate about violation of regulatory mechanisms, decrease in the number of T- lymphocytes, decrease of non-specific proliferative response to mitogens, increase of the B lymphocytes number. These observations led us to a deeper study of cells receptor system. The goal of the work was to identify rosetting and phagocytic activities of neutrophils in healthy and sick with infectious anemia horses in the dynamics of infection. Materials and methods: research of rosette forming phagocytic activities of horses neutrophils were determined using the guidelines of Busol V.A. etc. 43 horses of 10-12 years old were divided into three groups by the titer of antibodies to the EIA (Equine Infectious Anaemia) virus in the animal’s serum (19 - without antibodies, 20 - 1:2-1:8, 4 - 1:16-1:32). All horses were kept under the same conditions. The study was conducted in the fall season. Results of research and discussion. In healthy animals 89.7% neutrophils formed incomplete rosettes, with 3-4 erythrocytes, and 10.3% formed complete rosettes attaching 5 or more red blood cells. Comparing the group of sick animals (1:2-1:8, 1:16-1:32) with healthy, we found significant difference (р<0.01) in reducing the expression of full rosette receptors on the neutrophils. We have found significant difference (p<0.05) of increased expression of receptors for B-lymphocytes markers, which formed complete rosettes on neutrophils in sick horses with latent stage of EIA (1:16-1:32) compared with healthy animals. We also found that the level of rosette forming activity of neutrophils changed dynamically with the development of infection and changes in the receptors of these cells is more significant in the exacerbation of infection. Conclusions and prospects for further research: With the development of latent EIA infection changes of receptors expression to T- and B-lymphocytes markers are detected in neutrophils. Determined that the rosette forming activity for T-lymphocytes markers with exacerbation of infection was reduced and increased to the B-lymphocytes. Established inhibition of phagocytic activity of neutrophils, that confirmed by the number of captured bacterial cells and neutrophils participation in the process.
Authors and Affiliations
Pavel Kryvoshyja, L. Kot, Maxim Romanko, Oleg Rud
Keywords
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- EP ID EP270677
- DOI 10.31073/vet_biotech30-16
- Views 117
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