PHYTOCHEMICAL CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF THE NORTHERN MOROCCAN SPECIES: WITHANIA FRUTESCENS L.
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 6
Abstract
Objective: In this study, we were interested in qualitative, quantitative phytochemical characterization and evaluation of the antioxidant capacity of the total extracts of a plant from northern Morocco, the species selected for this study is Withania frutescens. Materials and Methods: Analysis of mineral elements by inductive coupling plasma-atomic absorption spectroscopy (ICP-AES), phytochemical screening, polyphenol and tannin assays, evaluation of antioxidant activity by the 1,2-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method and reducing power. Results: The plant contains alkaloids, saponins, tannins, mucilages, and coumarins. It has a relatively high content of total polyphenols and tannins of 19.53±0.018 μg genetic generalized epilepsy/mg MS and 6.258±0.062 μg Eqcat/mg MS, respectively. The analysis of mineral elements by ICP-AES shows that our species is rich in mineral elements which are calcium, magnesium, and sodium, and it is devoid of metallic elements such as nickel, lead, cadmium, and cobalt. The evaluation of antioxidant activity by the DPPH free radical scavenging method shows that the half maximal inhibitory concentration of the tested extracts has an antiradical activity of about 0.056±0.008 μg/ml for the ethanol extract and 0.213±0.004 μg/ml for the methanol extract compared to the butylated hydroxytoluene value of 0.009±0.0004 μg/ml which was used as a reference. The reducing capacity test shows that methanolic extract has a high antioxidant capacity (0.213±0.006) compared to ethanolic extract (0.043±0.004) but remains low compared to ascorbic acid (0.003±0.0004) which was used as reference. Conclusion: Phytochemical analysis of W. frutescens shows that this plant is rich in high quantities of alkaloids, saponins, mucilage, tannins, and coumarins. It contains an average amount of total polyphenols and tannins that confer significant antioxidant activity to the plant studied.
Authors and Affiliations
ABDELFATTAH EL MOUSSAOUI , FATIMA ZAHRA JAWHARI , DALILA BOUSTA , AMINA BARI
Keywords
Related Articles
- EP ID EP608996
- DOI -
- Views 133
- Downloads 0
POLYMORPHISM OF CYTOCHROME P450, SUPERFAMILY19, POLYPEPTIDE 1 GENE AND RELATED TO AROMATASE ENZYME ACTIVITY IN ACROMEGALY IRAQI PATIENTS
Objective: The objective of the present work was to investigate the association between aromatase enzyme activity and polymorphisms, rs2236722, in exon 2 of cytochrome P450 (CYP), superfamily19, polypeptide 1 (CYP19A1gen...
SYNTHESIS AND CHARACTERIZATION OF CYCLOHEXANE-1,3-DIONE DERIVATIVES AND THEIR IN SILICO AND IN VITRO STUDIES ON ANTIMICROBIAL AND BREAST CANCER ACTIVITY
Objective: The objective of this study was to evaluate in silico and in vitro anticancer activity for synthesized cyclohexane-1,3-dione derivatives. Methods: The new series of cyclohexane-1,3-dione derivatives were synt...
THE STUDY OF POTENTIALITY OF PICRORHIZA KURROA ROOT PROTEINS TO INHIBIT FREE RADICALS AND Α-AMYLASE ENZYME
 Objective: To prevent oxidative damage to cells caused by free radicals that are responsible for many diseases, such as atherosclerosis, diabetesand cancer. Picrorhiza kurroa (Katuki) is an ayurvedic medicinal plant us...
ALOE VERA: AN ASSURED WEIGHT LOSS DIET – AN APPROACH TOWARD IMPROVING THE JUICE PALATABILITY AND IN SILICO ANALYSIS
Objective: Obesity is an epidemic disease act as causative for global death. The principal aim of this study is to create an obesity treatment beverage that is palatable and readily acceptable to the public. Aloe vera ju...
Professor TOTAL PHENOLIC CONTENT AND FREE RADICAL SCAVENGING ACTIVITY OF MIMUSOPS ELENGI L. LEAVES
Objective: The motive of the current study was to find out the total phenolic content (TPC) and free radical scavenging (FRS) activity of various solvent extracts of leaves. Methods: Leaves powder was subjected to extra...