PHYTOCHEMICAL PROFILING USING LC-Q-TOF-MS ANALYSIS AND IN VITRO ANTIOXIDANT ACTIVITY OF A RARE SALT-SECRETING MANGROVE AEGIALITIS ROTUNDIFOLIA ROXB. LEAVES EXTRACT

Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2019, Vol 11, Issue 3

Abstract

Objective: The present work deals with the qualitative study of the phytoconstituents present in Aegialitis rotundifolia Roxb., ethanolic leaves extract and evaluate its antioxidant properties in vitro. Methods: The qualitative phytochemical analysis of the extract was performed first using preliminary phytochemical tests and then by liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS). The antioxidant properties were investigated comprehensively using seven in vitro models viz., 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, nitric oxide (NO) scavenging, hydrogen peroxide (H2O2) scavenging, superoxide (SOD) radical scavenging, lipid peroxidation (LPO) assay, reducing power (RP), and total antioxidant activity. Results: The preliminary phytochemical analysis revealed the presence of several important phytochemical groups whereas the LC-Q-TOF-MS analysis detected 25 phytoconstituents in the extract mostly belonging to flavonoids and alkaloids. The test extract showed strong dose-dependent antioxidant activity in all the seven in vitro models, however, the activity of the extracts was slightly lower compared to the reference standard ascorbic acid. Conclusion: The test extract showed strong antioxidant properties which could be possibly due to the phytoconstituents detected in the extract.

Authors and Affiliations

Debjit Ghosh, Sumanta Mondal, Prof. Karipeddi Ramakrishna

Keywords

Related Articles

ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF SELECTED MEDICINAL PLANTS AGAINST HUMAN ORAL PATHOGENS

Objective: The aim of the study was focused on determining the phytochemicals, antibacterial, antiadherence, antifungal and antioxidant activities of Glycyrrhiza glabra, Matricaria chamomilla and Eclipta alba and also th...

POTENTIAL CYTOTOXIC DRUG EFFECTS OF SECONDARY METABOLITES DERIVED FROM SELECTED MEDICINAL PLANTS OF SAVANADURGA FOREST IN KARNATAKA

Objective: To evaluate the potential cytotoxic and antitumor activities of secondary metabolites of selected medicinal plants of Savanadurga forest, Karnataka.Methods: The soxhlet extracted crude methanolic leaf extracts...

EVALUATION HYPOLIPIDEMIC ACTIVITY OF AROGYAVARDHINI AND ZPTER TABLET IN CHOLESTEROL-RICH HIGH FAT DIET (HFD) INDUCED HYPERLIPIDEMIA IN WISTAR RATS

Objective: The present research work aims to evaluate the hypolipidemic activity of arogyavardhini and zpter tablet in high fat diet (HFD) induced hyperlipidemia in wistar rats. Methods: Wistar rats were divided in 5 gr...

EVALUATION OF TAXIFOLIN AND PHLORETIN AS ANTIANGIOGENIC: AN IN VIVO, IN VITRO EXPERIMENTAL ANALYSIS

Objective: To examine the antiangiogenic effect of flavonoids (taxifolin and phloretin) against various In vivo and In vitro experimental models such as Chick Chorio Allantoic Membrane (CAM) assay, Dorsal Skinfold Chambe...

PHARMACOKINETICS AND BIOAVAILABILITY OF ORTHOSIPHON STAMINEUS ETHANOLIC EXTRACT AND ITS-NANO LIPOSOMES IN SPRAGUE-DAWLEY RATS

Objective: This study aimed to perform pharmacokinetic profile of rosmarinic acid (RA), sinensitin (SIN), eupatorin (EUP) and 3΄-hydroxy-5,6,7,4΄-tetramethoxyflavone (TMF) in Orthosiphon stamineus ethanolic extract (OS...

Download PDF file
  • EP ID EP565419
  • DOI 10.22159/ijpps.2019v11i3.29985
  • Views 170
  • Downloads 0

How To Cite

Debjit Ghosh, Sumanta Mondal, Prof. Karipeddi Ramakrishna (2019). PHYTOCHEMICAL PROFILING USING LC-Q-TOF-MS ANALYSIS AND IN VITRO ANTIOXIDANT ACTIVITY OF A RARE SALT-SECRETING MANGROVE AEGIALITIS ROTUNDIFOLIA ROXB. LEAVES EXTRACT. International Journal of Pharmacy and Pharmaceutical Sciences, 11(3), 37-47. https://europub.co.uk/articles/-A-565419