Plasmid Borne Resistance in Bacteria to Common Household Spices
Journal Title: Microbiology Research Journal International - Year 2015, Vol 5, Issue 2
Abstract
Aim: To examine the antimicrobial properties of various spices commonly used in Indian cuisine and to check if the resistance in bacteria is plasmid borne. Study Design: To demonstrate the antimicrobial properties in various household spices like Cinnamon (Cinnamomum zeylanicum), Clove (Syzygium aromaticum), Black Pepper (Piper nigram) and Turmeric (Curcuma longa) and to examine their effects on the pathogenic bacteria Escherichia coli, Bacillus subtilis, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. Place and Duration of Study: Department of Microbiology, Osmania University, India during June 2013- January 2014. Methodology: Antimicrobial assay was performed to determine the antimicrobial activity of these spices. Plasmids were isolated from bacteria showing resistance to these spices to confirm if the resistance to these spices was plasmid borne. The isolated plasmids were used to transform E. coli DH5α which was previously observed to be sensitive to these spices. Results: On transformation with plasmids isolated from K. pneumoniae and P. aeruginosa, E. coli DH5α cells were observed to gain resistance to all the four spices tested in the original strains of K. pneumoniae and P. aeruginosa. Conclusion: The resistance to spices seems to be a plasmid borne feature which may be transferred to sensitive bacterial strains.
Authors and Affiliations
Anoor Pawan Kumar, Karthik Rajkumar, M. Swathi Raju, A. Nichita
Keywords
Related Articles
- EP ID EP354572
- DOI 10.9734/BMRJ/2015/11066
- Views 85
- Downloads 0
Association of Multiple Sclerosis with Epstein-Barr virus Infection
Purpose of Review: There is still controversy whether EBV could be a causative agent as opposed to an innocent bystander in the pathogenesis of multiple sclerosis (MS). We aimed to review main studies which were investig...
Isolation and Identification of Potential Gossypol Degrading Fungal Strains from Cotton Growing Soil
Aim: To isolate and identify gossypol degrading fungal strains from cotton growing soil of Maharashtra and Andhra Pradesh States of India. Study Design: Experimental study. Place and Duration of the Study: Chemical and...
Isolation and Molecular Detection of Pathogenic Vibrio Species among Economic Fish from Red Sea in Egypt
A total of 105 samples were collected from Siganus rivulatus, Mulloidichthys vanicolensis, and Lethrinus lentjan, freshly captured from the Red Sea along Hurghada City coastline zone, Egypt. Clinical and post mortem find...
Community-Acquire CTX-M Beta-Lactamase Enteric Isolates in Abeokuta, Nigeria
Aim: To study the emergence of the community-acquire CTX-M beta-lactamase genes among the residents in Abeokuta, Nigeria. Study Design: To determine the prevalence rate of CTX-M beta-lactamase genes in enteric multi-resi...
Antibiotic Susceptibility Test of Klebsiella pneumoniae and K. oxytoca Isolated from Different Clinical Samples and Perform Random Amplified Polymorphic DNA among K. pneumoniae
Aims: To study the prevalence of MDR and ESBL producing Klebsiella pneumoniae and Klebsiella oxytoca and perform RAPD (Random amplified polymorphic DNA) by optimizing PCR among isolated ESBL producing Klebsiella pneumoni...