Platelet-rich plasma collection and preparation: a retrospective analysis of 256 cases
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 10
Abstract
Objective To retrospectively analyze the safety and product quality of NGL XCF 3000 blood cell separator for collecting platelet-rich plasma (PRP) in 256 cases, so as to provide reference for safe collection and product quality control of PRP. Methods The data of 256 patients receiving PRP treatment in our hospital from June 2021 to June 2022 were statistically analyzed, and the differences in the collection time, circulating blood volume and the occurrence of adverse reactions to blood donation were analyzed when NGL XCF 3000 was used to collect autologous PRP among patients of different genders, ages and platelet counts. The differences in platelet content, red blood cell(RBC) contamination and white blood cell(WBC) residues in PRP products were analized. Results 1) There were no significant differences in collection time, circulating blood volume and collection volume among patients of different genders, ages and platelet counts (P<0.05). 2) The contents of WBC, RBC and platelet were not significantly different between male and female patients after collection (P<0.05); 3) The WBC contents increased with the increase of age, and the WBC residue in the elder group[ 56 to78 years old, (0.64±0.41) ×109/L] was significantly higher than that in the younger group[group 1,18 to 40 years old, (0.50±0.35)×109/L], with significant difference was Statistically significant (P<0.05). 4) The residues of WBCs and RBCs in in low platelet group [group 1, (100-150)×109/L] were higher than those in other platelet count groups, and the difference was Statistically significant (P<0.05), and the platelet count in this product was significantly lower than that in other platelet count groups (P<0.05). Conclusion The NGL XCF 3000 blood cell separator is safe and stable for PRP collection in patients with different genders, ages and platelet counts of (100-450)×109/L, and the PRP products collected can meet clinical therapeutic needs.
Authors and Affiliations
Lijuan YANG, Yan HU, Yajuan WU, Li ZHANG, Liping LIU, Tao PENG, Xinyu GAN
Keywords
Related Articles
- EP ID EP738672
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.10.021
- Views 68
- Downloads 0
The establishment and application of anemergency recruitment strategy for blood donors in Shenzhen under public health emergencies
Objective To establish and improve the emergency recruitment strategy of voluntary blood donors under public health emergencies in Shenzhen and verify its effectiveness. Methods Blood donor recruitment strategies under r...
Adverse events in platelet-rich plasma collection and countermeasures
Objective To explore the quality of platelet-rich plasma(PRP) and adverse events during PRP collection and the countermeasures, so as to provide reference for the development of PRP therapy. Methods A total of 412 patien...
Impact of HLA antigen and antibody expression on cross-matching and transfusion effect
[Objective] To study the relationship between the expression intensity of HLA-Ⅰ platelet antibodies in patients with platelet transfusion refractoriness (PTR) and platelet cross-matching, and to further evaluate other fa...
HBV serologic and virus characteristics of HBsAg negative and NAT non-discriminated samples from blood donors
Objective To investigate the HBV infection of TMA initially reactive but discriminatory test non-reactive samples(NDR) after the individual donation nucleic acid detection(ID-NAT)of TMA, and analyze its serological and m...
B/O blood group chimera identified by PacBio third-generation sequencing: a case report
[Objective] To determine the blood group of a patient with ABO forward and reverse typing discrepancies using PacBio third-generation sequencing (TGS) technology, and to explore the application of serological methods and...