Platelet-rich plasma combined with gypenosides for the treatment of psoriasis-like inflammation in mice
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 12
Abstract
[Objective] To investigate the therapeutic effect of platelet-rich plasma (PRP) subcutaneous injection combined with gypenosides (GPs) oral administration on BALB/c mouse psoriatic inflammation and explore its mechanism of action. [Methods] The 6-8 week-old female SPF BALB/c mice were randomly divided into five groups: control, model, PRP, GPs and PRP+GPs group, with 5 mice in each group. Imiquimod (IMQ) was used to induce psoriasis-like skin inflammation on the back of mice except the control group. The onset and severity of psoriasis-like inflammation in different treatment groups were evaluated by observing skin lesions, skin thickness and measuring PASI score. HE staining and Ki67 staining were used to evaluate the pathological changes and proliferation of keratinocytes in psoriasis-like skin lesions. Blood cell count, enzyme-linked immunosorbent assay and Western blot were used to explore the changes in circulating white blood cell count, cytokines IL-17A and TNF-α, and related signaling pathway proteins p-STAT3 and p-P38. [Results] At the end of the experiment (on day 6), scale scores of model, PRP, GPs and PRP+GPs group were 3.6±0.49, 1.8±0.75, 1.8±0.75, 1.2±0.40, respectively; the ratios of skin thickness (μm) were 0.86±0.18, 0.59±0.10, 0.56±0.07 and 0.42±0.09; PASI scores were 10.6±1.02, 4.0±0.63, 4.0±1.10 and 3.2±0.75. Compared with the model group, the number of scales (P<0.01), patch thickness (P<0.01) and PASI score decreased (P<0.0001) showed a certain therapeutic effect, and PRP+GPs group had the best effect. Pathological examination showed that both the epidermal layer thickness (P<0.01) and epidermal cell proliferation (P<0.05) decreased in all treatment groups; IL-17A expression levels were 9.02±2.54, 16.56±3.49, 10.01±1.83, 11.12±2.48 and 10.50±2.16, and TNF-α expression levels were 223.36±70.34, 377.36±58.47, 265.42±45.14, 262.94±33.29 and 268.94±26.80 respectively. The expression of skin tissue IL-17A (P<0.05) and TNF-α (P<0.05) decreased, along with the decreased expression of related signaling pathway proteins p-STAT3 and p-P38. [Conclusion] PRP combined with GPs can reduce the expression of IL-17A and TNF-α through the STAT3 and P38 signaling pathways, thereby alleviating inflammation and inhibiting the overproliferation of keratinocytes, thus improving psoriasis-like skin inflammation in BALB/c mice.
Authors and Affiliations
Dandan LI, Bing WANG, Yu GE, Hong CHENG, Mengxue LI, Zhicheng WANG, Rong XIA
Keywords
Related Articles
- EP ID EP754449
- DOI 10.13303/j.cjbt.issn.1004-549x.2024.12.002
- Views 18
- Downloads 0
Correlation between serological screening of human T-lymphotropic virus antibodies and confirmatory tests
Objective To explore the correlation between serological screening of human T-lymphotropic virus antibodies (anti HTLV) and Western blot(WB) confirmatory tests among blood donors, so as to explore the infection status of...
Establishment of a method for detecting complement C3d-sensitized platelets
[Objective] To establish a detection method for complement C3d-sensitized platelets. [Methods] Parallel detection of the same platelet sample under conditions of complement C3d sensitization and non-sensitization was con...
Application of therapeutic hemapheresis and the adverse reactions
Objective To discuss the application of therapeutic hemapheresis in clinical therapy and to observe the adverse reactions. Methods 126 times of therapeutic hemapheresis were performed in 121 patients by Nangel blood cell...
Research progress and future of red blood cell substitutes
This paper briefly reviewed blood substitutes, focusing on the background, history of research and development(R&D), types, functions, characteristics, the indications that will be mainly targeted in clinical, and their...
Risk factors of red blood cell infusion in very low/ultra-low birth weight neonates with respiratory distress syndrome
Objective To investigate the risk factors of red blood cell transfusion frequency (fRBCT) toward newborns with very/extremely low birth weight (V/ELBW) who experienced <32 weeks of gestational age and were complicated wi...