Polyphenolic compounds (HPLC analysis) and Antioxidant Activity of Stevia Rebaudiana (Asteraceae) by FRAP and DPPH Assay in greenhouse and free space condition
Journal Title: International Journal of Farming and Allied Sciences - Year 2017, Vol 6, Issue 2
Abstract
This paper aimed to establish the content in polyphenolic compounds and antioxidant activity of Stevia Rebaudiana (Asteraceae) by FRAP and DPPH Assay in greenhouse and Free space condition. A high-performance liquid chromatographic method coupled with UV and fluorescence (HPLC) detectors for the determination of polyphenols in Stevia samples are reported. Hesperedin, Ellagic acid, Rosmarinic acid, Chloregenic acid, Eugenol, Coumarin and Vanilin were detected of Stevia. Samples were injected into the chromatograph directly without previous treatment. In the second part of the work the antioxidant activity of the samples were determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and Ferric ion reducing activity(FRAP) assay. The antioxidant activities of greenhouse essential oil were stronger than those of the essential oil of free space condition with regard to DPPH radical scavenging and FRAP. The correlation between specific polyphenols and the antioxidant activity in samples was also investigated. FRAP assay showed better antioxidant activity than DPPH assay. The FRAP assay, is presented as a novel method for assessing “antioxidant power.” The assay is inexpensive, reagents are simple to prepare, results are highly reproducible, and the procedure is straightforward and speedy.
Authors and Affiliations
Sharareh Najafian, Mojtaba Moradi
Keywords
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