Polyphenolic compounds (HPLC analysis) and Antioxidant Activity of Stevia Rebaudiana (Asteraceae) by FRAP and DPPH Assay in greenhouse and free space condition
Journal Title: International Journal of Farming and Allied Sciences - Year 2017, Vol 6, Issue 2
Abstract
This paper aimed to establish the content in polyphenolic compounds and antioxidant activity of Stevia Rebaudiana (Asteraceae) by FRAP and DPPH Assay in greenhouse and Free space condition. A high-performance liquid chromatographic method coupled with UV and fluorescence (HPLC) detectors for the determination of polyphenols in Stevia samples are reported. Hesperedin, Ellagic acid, Rosmarinic acid, Chloregenic acid, Eugenol, Coumarin and Vanilin were detected of Stevia. Samples were injected into the chromatograph directly without previous treatment. In the second part of the work the antioxidant activity of the samples were determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and Ferric ion reducing activity(FRAP) assay. The antioxidant activities of greenhouse essential oil were stronger than those of the essential oil of free space condition with regard to DPPH radical scavenging and FRAP. The correlation between specific polyphenols and the antioxidant activity in samples was also investigated. FRAP assay showed better antioxidant activity than DPPH assay. The FRAP assay, is presented as a novel method for assessing “antioxidant power.” The assay is inexpensive, reagents are simple to prepare, results are highly reproducible, and the procedure is straightforward and speedy.
Authors and Affiliations
Sharareh Najafian, Mojtaba Moradi
Keywords
Related Articles
- EP ID EP32887
- DOI -
- Views 360
- Downloads 0
Water consumptive use of Radish (Rhapanus sativus L. Var sativus), as influenced by varying temperatures
Seeds of Topsi, Famox F1, Corox F1, and Altox F1 radish cultivars germinated in controlled 20 and 12oC cabinets. The objective of this experiment was to determine water consumptive use, water use efficiency and irrigat...
Identification of chemical compounds and antimicrobial effects of essential oils of Artemisia scoparia and A. Aucheri
Using of antimicrobial compositions from medicinal plants against bacteria is growing up with respect to resist the bacteria in relation to derived antibiotics from microorganisms. Hence, identification of chemical com...
The study of the effects of mycorrhiza and potassium fertilizer on certain characteristics of the coriander plant under salt stress conditions
Salt stress is one of the most important abiotic stresses in the world, whose negative effects on the crop development has increased the number of researches on the salt tolerance, aimed to improve the plant tolerance....
Effect of planting patterns on yield and some agronomical traits in saffron (Crocus sativus L.) Under different irrigation intervals in Shahr-e-Rey Region
Due to study the effect of planting patterns on yield and some agronomical traits in saffron (Crocus sativus L.) under different irrigation intervals, an experiment was done in summer 2010 at Shahr-e-Rey agricultural r...
Effect of planting pattern and cultivar on some quantitative characteristics of canola
The seed yield of oilseed rape is a function of population density, number of pods per plant, number of seeds per pod and seed weight. However, yield structure is very plastic and adjustable across a wide range of popu...