Abstract

BACKGROUND: Screening blood donors has practically eliminated viral and bacterial pathogen in blood used for transfusion. However, transfusion associated bacterial sepsis remains an important health care concern and the commonest cause of transfusion related fatality in resource limited settings. Data on bacterial contamination of blood are scare while the demand of blood transfusion is continuously growing .Thereafter we conducted a study to determine the prevalence and type of bacterial contamination in donor blood and blood products at the S.S.G.Hospial, blood bank, Vadodara. MATIREAL & METHOD: A cross sectional study was conducted at blood bank S.S.G.Hospial, Vadodara between march 2016 to may 2016.A total of 148 units of screen blood and blood products consisting of refrigerated whole blood and packed red cells were randomly sampled following aseptic procedures. Samples from each unit were collected in universal containers containing brain heart infusion broth and incubated at 37degree Celsius for upto 7 day .subculture were carried out on blood agar, chocolate agar and Mckconkey agar in microbiology laboratory S.S.G.Hospital.Isolates were identified by standard microbiologic techniques. RESULT: Of the 148 samples collected 5(3.37%) samples were showed growth. The contaminates were Staphylococcus aureus, Klebsella spp. and Coagulase Negative Staphylococcus were the common isolates identified. In similar studies by Makuni et al from National Blood Service, Zimbabwe and Wondimu et al from University of Gondar Hospital Blood Bank, Northwest Ethiopa the results for bacterial contamination were 2/149(1.3%) and 21/137(15.3%) respectively. Our study showed bacterial prevalence higher than Makuno et al study while lower than the study by Wondimu et al. CONCLUSION: The Prevalence of bacterial contamination in blood products suggests the patient who receives blood products are at risk of developing infection. Therefore, it is critical to improve hygiene precaution in order to minimize bacterial contamination and ensure patient safety.

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