Prevalence of blaTEM, blaSHV and blaCTX-M among β-lactamase producing Escherichia coli isolated from tertiary care hospitals in Taif, Saudi Arabia
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 1
Abstract
Seventy clinical isolates of Escherichia coli were obtained from two tertiary care hospitals in Taif Governorate, Saudi Arabia and investigated for their susceptibility to 15 antibiotics of different classes using antibiotic disc diffusion method. Amikacin and imipenem were the most active antibiotics while, ampicillin was the least active. Multiple drug resistance was detected in 52.9% of the isolates which were resistant to 3-6 antibiotics of three or more different classes. All isolates were tested for their ability to produce β-lactamases in general and extended spectrum β-lactamase in particular by iodometric overlay method using penicillin, cefotaxime, ceftazidime and cefepime as a substrates. It was found that 67%, 38.6%, 20% and 40% of isolates were found to degrade the previous substrates respectively. On the basis of CLSI criteria, 57.1% of the isolates were phenotypically categorised as ESBLs. On the other hand, molecular investigation of β-lactamases revealed that 54.3%, 7.1% and 10.0% of isolates were found to harbour blaTEM, blaSHV and blaCTX-M genes respectively. More strict legislations for the use of antibiotics are suggested to avoid spread and emergence of antibiotic resistance.
Authors and Affiliations
Ahmed A. AlBarraq, Mohamed F. El-Badawy2,5, Mohamed A. Al-Qarni, Samy S. Al-Saedi, Wael M. Al-Sufyani, Fahd E Osaimi4 ,, Mohamed M. Shohayeb5,
Keywords
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- EP ID EP388225
- DOI 10.9790/3008-1201031116
- Views 43
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