PREVALENCE OF EXTENDED SPECTRUM BETA-LACTAMASES AND AMP-C BETA-LACTAMASES IN CLINICAL ISOLATES OF GRAM-NEGATIVE BACILLI AT A TERTIARY CARE HOSPITAL
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2018, Vol 7, Issue 39
Abstract
BACKGROUND Amp-C beta-lactamase are cephalosporinases that hydrolyze cephamycins as well as other extended spectrum cephalosporins and are poorly inhibited by clavulanic acid. Although, reported with increasing frequency, the true rate of occurrence of Amp-C βlactamases in different organisms including members of Enterobacteriaceae remains unknown. The present study was designed to determine the occurrence of Extended Spectrum Beta-Lactamases (ESBL) and Amp-C betalactamase enzyme harbouring Gram-negative clinical isolates in a tertiary care hospital in South India. MATERIALS AND METHODS A total of 205 Gram-negative bacteria were screened for ESBL and Amp-C beta-lactamases. Isolates found to be positive by the screening test were subjected to the confirmatory test for ESBL and Amp-C disk test for plasmid Amp-C production. RESULTS Of the 205 clinical isolates, 135 (65.9%) were screen test positive for ESBL. Confirmatory test for ESBL revealed 79 (38.5%) isolates to be positive and 71 isolates were screen test positive for Amp-C beta-lactamase. Plasmid Amp-C was detected in 32 (15.6%) by Amp-C disc test. Co-production of ESBL and Amp-C was seen in 9 (4.4%) isolates and maximum coexistence was seen in Klebsiella pneumoniae (15.3%) isolates. CONCLUSION Screening and confirmatory tests are necessary to identify ESBL and true Amp-C producers, as it is a simple and rapid procedure. The high incidence of beta-lactamase production due to multiple mechanisms is alarming and requires urgent action from both a therapeutic and infection control perspective, so that proper antibiotic policy and appropriate measures could be taken to restrict the indiscriminate use of cephalosporins and carbapenems, so as to minimise the emergence of this multiple beta-lactamase producing organisms.
Authors and Affiliations
Malini Jagannatha Rao, Shruthi Harle, Padmavathy M
Keywords
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- EP ID EP548145
- DOI 10.14260/jemds/2018/953
- Views 81
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