Proteomic Analysis of the Immunosuppressive Effects of Mesenchymal Stem Cells in a Rat Heart Transplantation Model
Journal Title: Advances in Clinical and Experimental Medicine - Year 2013, Vol 22, Issue 6
Abstract
<strong>Background. </strong>Some reports suggest mesenchymal stem cells (MSCs) have immunosuppressive properties. However, conflicting evidence regarding the role of MSCs has emerged.<br /><strong>Objectives. </strong>To gain a better understanding of the immunosuppressive properties of mesenchymal stem cells (MSCs) in a rat heart transplantation model.<br /><strong>Material and Methods. </strong>MSCs were obtained from the femoral and tibial bone marrow of Sprague-Dawley rats and cultured. Heart-transplanted rats were allocated into a MSC-treated group and 2 control groups. On postoperative day 7, 1 rat was sacrificed and the pathological changes of heart tissues were assessed. Serum proteomic spectra were generated by surface-enhanced laser desorption/ionization-time-of-flight mass spectrometry (SELDI-TOF-MS).<br /><strong>Results. </strong>Rat MSCs displayed the typical spindle-shaped morphology in culture and significantly prolonged the graft survival up to 33.25 ± 2.54 days compared with controls (19.75 ± 1.56 and 11.16 ± 1.34 days, respectively). Pathological analysis showed the inflammatory cell infiltration in the MSC-treated group was significantly reduced. SELDI analysis showed that 5 protein/peptide peaks with M/Z 1272.33, 1986.65, 2323.42, 5375.59 and 12968.11 were up-regulated in the MSC-treated group (<em>P </em>< 0.001).<br /><strong>Conclusions. </strong>Donor-derived MSCs clearly alleviate acute rejection following heart transplantation in rats and significantly prolong the isograft survival time.
Authors and Affiliations
Shuo-Ming Wu, Wen-Xiong Zhang, Ming-Hui Wang, Hui-Zhong Zhang, Duo-Guang Wu, Zhi-Juan Zhou, Li-Hua Xiong
Keywords
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