Protocol for the artificial inoculation of plants of Musa spp. with Mycosphaerella fijiensis and evaluation of its response using epiphytological variables and components of the resistance
Journal Title: Biotecnología Vegetal - Year 2010, Vol 10, Issue 2
Abstract
The use of assessment methods of Musa response to natural infection with M. fijiensis in field has allowed differentiation of susceptible, partially resistant and resistant genotypes to black Sigatoka. However, artificial inoculation methods are not reproducible. Neither, characterization of the infective cycle of M. fijiensis combined with the use of epidemiological variables and components of resistance to differentiate genotypes of Musa spp has been achieved. A protocol to solve this problem is presented in this paper. It describes aspects related to cultural characteristics, morphological, molecular and aggressiveness of isolates of M. fijiensis, and the use of descriptive evaluation scale. It also allows the use of epidemiological variables and components of resistance to characterize the infective cycle of M. fijiensis in greenhouse and differentiate the response of susceptible and resistant genotypes. This protocol is able to reduce the time required to evaluate the resistance of Musa spp against M. fijiensis and therefore to make a greater number of evaluations. It also provides a tool for supporting related studies on Musa-M. fijiensis interaction, facilitates the validation of bioinformatic predictions, opens a path in search of new candidates of fungicides and biological control of black Sigatoka.
Authors and Affiliations
Michel Leiva-Mora , Yelenys Alvarado-Capó, Mayra Acosta-Suárez, Mileidy Cruz-Martín, Cynthia Sánchez-García, Berkis Roque
Keywords
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